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4 Results for "novus"

NB120-19294

LAMP-1/CD107a Antibody

(2 Reviews)

(33 Publications)

100 μg

NB120-19294

Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Analysis of 20 ug of HeLa cell lysate and 5 ul of Molecular Weight Protein Ladder per well.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin D (CathD) expression and localization in primary neurons. Colocalization of transfected CathD with LAMP-1/CD107a. CathD transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathD. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Lamp1 (red) in HeLa cells.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin B (CathB) expression and localization in primary neurons. Colocalization of transfected CathB with LAMP-1/CD107a. CathB transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathB. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.

EsxG-EsxH alters phagosomal GAL3, ubiquitin, and LAMP1. (A, C, and E) IF images of GAL3 (A), ubiquitin (FK2 antibody) (C), and LAMP1 (E) in BMDMs that were infected with DsRed-expressing H37Rv (WT) or the deltaesxH mutant for 3 h. Images are maximum-intensity projections. Scale bar, 10 um. Boxed areas in the merged image are shown in higher magnification in the rightmost panel. Mtb, M. tuberculosis. (B, D, and F) Automated image analysis was used to quantify the MFI of GAL3 (B), ubiquitin (D), and LAMP1 (F) colocalized with individual bacilli from 5 fields of a 12-mm coverslip. Data are means +/- SEM from one representative experiment from three (A, B, E, and F) or two (C and D) independent experiments. ****, P <= 0.0001, Student's t test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30482832), licensed under a CC-BY licence.

Inhibition of H4K12ac with NU9056 modulates cytokine and chemokine production. After 5–7 days of differentiation, MDDCs were treated with NU9056 50 nM, 0.2% EtOH or both for 5 days. Supernatants collected were analyzed for 48 inflammatory cytokines and chemokines. Data is from 3 blots for untreated control, 2 blots each for treatment with 0.2% EtOH alone, treatment with NU9056 alone, and treatment with 0.2% EtOH plus NU9056. Cytokines chosen to be presented in the graph were selected on the basis of fold change (2 folds or more) compared to control. Each cytokine is detected in duplicates within each blot. Statistical differences were calculated using student’s t-test when individually compared to untreated control and indicated with a * (compared to control) and # (between treatments) for significant p value. Two-way ANOVA was carried out (p = 0.027). Panel a shows optical density values for cytokines IL-15, RANTES, TGF-beta 1 and TNF-alpha . Panel b shows optical density values for MCP2. In panel c, MCP-2 ELISA was carried out from cell culture medium at least three times in duplicates. Values detected in pg/mL are as follows, untreated control (322.4 ± 25.04), NU9056 50 nM (317.5 ± 14.3), 0.2% EtOH (401.7 ± 28.2) and 0.2% EtOH + NU9056 50 nM (321.6 ± 13.5, p = 0.03). Panels d–g show intracellular staining to measure MCP-2 levels in untreated or treated MDDCs. Data represented is from at least 3 experiments. Panel d shows % gated MCP-2 positive cells for untreated control (52.3% ± 2.3), NU9056 50 nM (45.8% ± 1.07), 0.2% EtOH (85.8% ± 5.2, p = 0.002) and 0.2% EtOH + NU9056 50 nM (29.6% ± 1.18, p = 0.0001). Panel e shows MFI values for MCP-2-FITC for untreated control (2510 ± 29), NU9056 50 nM (2151 ± 43), 0.2% EtOH (4260 ± 603, p = 0.04) and 0.2% EtOH + NU9056 50 nM (1459 ± 297, p = 0.01). Panel f shows an overlay histogram of MCP-2-FITC for all the samples as shown in legend. Panel g shows representative single cell images. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41598-017-11172-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

DDR and NF-kappa B are activated concomitantly in senescent MEFs and aged tissues. (A) Immunoblot detection of p-p65 and total p65 in liver tissue from 16-week-old WT (n=3) and Ercc1-/ delta (n=3) mice. (B) Immunoblot detection of phosphorylation of ATM and downstream targets gamma H2AX and p21 in liver from 16-week-old WT and Ercc1-/ delta mice. (C) Immunoblot detection of phosphorylation of NF-kappa B and I kappa B alpha in liver lysates from 3, 12 and 24 month-old WT mice. n=3 mice per group. (D) Immunoblot detection of p-ATM, ATM and p21 in the same liver lysates. (E) Immunoblot detection of DDR effectors in nuclear extracts from passage 5 WT and Ercc1-/- MEFs, grown at 20% oxygen. (F) Level of NF-kappa B activation is higher in Ercc1-/- MEFs compared to WT MEFs at passage 5, as measured by Immunoblot detection of p-p65 and total p65 in WT and Ercc1-/- MEFs at passage 5 after culturing in 20% oxygen. (G) Representative images of immunofluorescent detection of p65 and NEMO in passage 4 WT and Ercc1-/- MEFs grown at 20% oxygen. Blue: DAPI staining; Green: p65 (top panel) or NEMO (bottom panel). Images were taken at the magnification of 60x. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32201398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-19294AF350
Alexa Fluor 405	NB120-19294AF405
Alexa Fluor 488	NB120-19294AF488
Alexa Fluor 532	NB120-19294AF532
Alexa Fluor 594	NB120-19294AF594
Alexa Fluor 647	NB120-19294AF647
Alexa Fluor 700	NB120-19294AF700
Alexa Fluor 750	NB120-19294AF750
Allophycocyanin	NB120-19294APC
Biotin	NB120-19294B
DyLight 350	NB120-19294UV
DyLight 405	NB120-19294V
DyLight 488	NB120-19294G
DyLight 550	NB120-19294R
DyLight 594	NB120-19294DL594
DyLight 650	NB120-19294C
DyLight 680	NB120-19294FR
DyLight 755	NB120-19294IR
FITC	NB120-19294F
HRP	NB120-19294H
Janelia Fluor 549	NB120-19294JF549
Janelia Fluor 646	NB120-19294JF646
PE	NB120-19294PE
PerCP	NB120-19294PCP
mFluor Violet 450 SE	NB120-19294MFV450
mFluor Violet 500 SE	NB120-19294MFV500
mFluor Violet 610 SE	NB120-19294MFV610

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AF4320

LAMP-1/CD107a Antibody [Unconjugated]

(2 Reviews)

(16 Publications)

100 μg

AF4320

Western blot shows lysates of mouse small intestine tissue and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4320-SP

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AF4800

LAMP-1/CD107a Antibody [Unconjugated]

(1 Review)

(9 Publications)

100 μg

AF4800

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LAMP-1/CD107a at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LAMP1/CD107a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using 10 µg/mL Sheep Anti-Human LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	IC7985G
Biotin	BAF4800

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC7985G
Carrier Free	AF4800-SP

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NBP2-59780

LAMP-1/CD107a Antibody (CL3484)

(1 Review)

100 ul

NBP2-59780

Western Blot: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human liver shows strong granular cytoplasmic immunoreactivity in hepatocytes.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human fallopian tube shows granular cytoplasmic positivity in epithelial cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human endometrium shows granular cytoplasmic immunoreactivity in epithelial and stromal cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human prostate shows strong granular cytoplasmic positivity in epithelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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