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![Western Blot: GOT2 Antibody [NBP2-16708] - 30 ug Neuro2A whole cell lysate/extract 10 % SDS-PAGE GOT2 antibody dilution: 1:1000. (Mouse)](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP2-16708-img0008.jpg "Western Blot: GOT2 Antibody [NBP2-16708] - 30 ug Neuro2A whole cell lysate/extract 10 % SDS-PAGE GOT2 antibody dilution: 1:1000. (Mouse)")
![Immunocytochemistry/Immunofluorescence: GOT2 Antibody [NBP2-16708] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16708-img0007.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Mouse brain. GOT2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP2-16708-img0011.jpg)
![Western Blot: GOT2 Antibody [NBP2-16708] - 50 ug rat lung lysate/extract 10 % SDS-PAGE GOT2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP2-16708-img0002.jpg)
![Western Blot: GOT2 Antibody [NBP2-16708] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP2-16708-img0003.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Hepatoma tissue. GOT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP2-16708-img0004.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - RT2 xenograft, using GOT2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP2-16708-img0006.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Mouse liver. GOT2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP2-16708-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: GOT2 Antibody [NBP2-16708] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP2-16708-img0012.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Analysis in human liver and bone marrow tissues. Corresponding GOT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-81565-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Analysis in human liver and bone marrow tissues. Corresponding GOT2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GOT2 Antibody [NBP1-81565] - Analysis in human cell lines PC-3 and SK-MEL-30. Corresponding GOT2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP1-81565-img0013.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human liver shows very strong cytoplasmic granular positivity in hepatocytes.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-81565-img0019.jpg)
![Western Blot: GOT2 Antibody [NBP1-81565] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP1-81565-img0006.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human bone marrow shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-81565-img0015.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human cerebellum shows strong cytoplasmic granular positivity in cells in granular layer.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-81565-img0016.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human testis shows strong cytoplasmic granular positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-81565-img0018.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human small intestine shows moderate cytoplasmic granular positivity in glandular cells.](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-81565-img0017.jpg)
![Western Blot: GOT2 Antibody [NBP2-32241] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GOT2 antibody NBP2-32241 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP2-32241-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: GOT2 Antibody [NBP2-32241] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GOT2 antibody NBP2-32241 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-NBP2-32241-img0003.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-NBP2-32241-img0002.jpg)
![Western Blot: GOT2 Antibody (3E9) [NBP1-47469] - Analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/GOT2-Antibody-3E9-Western-Blot-NBP1-47469-img0007.jpg "Western Blot: GOT2 Antibody (3E9) [NBP1-47469] - Analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: GOT2 Antibody (3E9) [NBP1-47469] - Analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/GOT2-Antibody-3E9-Immunocytochemistry-Immunofluorescence-NBP1-47469-img0008.jpg)
![Western Blot: GOT2 Antibody (4J7O3) [NBP3-15708] - Analysis of extracts of various cell lines, using GOT2 antibody (NBP3-15708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/GOT2-Antibody-4J7O3-Western-Blot-NBP3-15708-img0003.jpg "Western Blot: GOT2 Antibody (4J7O3) [NBP3-15708] - Analysis of extracts of various cell lines, using GOT2 antibody (NBP3-15708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: GOT2 Antibody (4J7O3) [NBP3-15708] - Human liver using GOT2 Rabbit mAb (NBP3-15708) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GOT2-Antibody-4J7O3-Immunohistochemistry-Paraffin-NBP3-15708-img0002.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody (4J7O3) [NBP3-15708] - Rat brain using GOT2 Rabbit mAb (NBP3-15708) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GOT2-Antibody-4J7O3-Immunohistochemistry-Paraffin-NBP3-15708-img0001.jpg)
![Western Blot: GOT2 Antibody (SR1734) [NBP3-22345] - Western blot analysis using NBP3-22345 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22345_rabbit-got2-mab-sr1734-57202315521838.jpg "Western Blot: GOT2 Antibody (SR1734) [NBP3-22345] - Western blot analysis using NBP3-22345 in HepG2 cell lysate.")
![Western Blot: GOT2 Antibody [NB100-93404] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NB100-93404-img0002.jpg "Western Blot: GOT2 Antibody [NB100-93404] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: GOT2 Antibody [NBP1-00195] - Western blot analysis of GOT2 (aa 360 to 373) in Human Liver cell lysate (35ug protein in RIPA buffer) using NBP1-00195 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP1-00195-img0003.jpg "Western Blot: GOT2 Antibody [NBP1-00195] - Western blot analysis of GOT2 (aa 360 to 373) in Human Liver cell lysate (35ug protein in RIPA buffer) using NBP1-00195 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
