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![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunofluorescence-NBP2-76807-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).")
![Flow Cytometry: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Flow-Cytometry-NBP2-76807-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunofluorescence-NBP2-76807-img0002.jpg)
![Western Blot: Ataxin-2 Antibody [NB100-58797] - Detection of Human and Mouse ATX2/SCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Western-Blot-NB100-58797-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ataxin-2 Antibody [NB100-58797] - Detection of Human and Mouse ATX2/SCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: Ataxin-2 Antibody [NB100-58797] - Detection of human ATX2/SCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for IP at 6 ug/mg lysate. ATX2/SCA2 was also immunoprecipitated by rabbit anti-ATX2/SCA2 antibody from Company B. For blotting immunoprecipitated ATX2/SCA2, NB100-58797 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunoprecipitation-NB100-58797-img0004.jpg)
![ELISA: Mouse Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69898] - Samples were spiked with high concentrations of Mouse Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69898_mouse-ataxin-2-elisa-kit-colorimetric-13220241681499.jpg "ELISA: Mouse Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69898] - Samples were spiked with high concentrations of Mouse Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69898] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69898_mouse-ataxin-2-elisa-kit-colorimetric-432024915169.jpg)