| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1182SS | |||
| Azide and BSA Free | NBP2-80721 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22381SS | |||
| Azide and BSA Free | NBP2-80722 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44643-0.02mg | |||
| Azide and BSA Free | NBP2-47876-0.1mg | |||
| IHC-Prediluted | NBP2-48288 | |||
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Immunohistochemistry-Paraffin-NB600-1182-img0009.jpg "Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.")
![Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Western-Blot-NB600-1182-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-EpCAM/TROP1 (AUA1) conjugated to Alexa Fluor 488 [NB600-1182AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Immunocytochemistry-Immunofluorescence-NB600-1182-img0016.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue with 1:500 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The staining was primarily localized to the membranes and inter-cellular spaces of the cancer cells. Staining intensity for 1:500 dilution was lower than what was seen in sections tested with 1:100 dilution. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Immunohistochemistry-Paraffin-NB600-1182-img0014.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Using the PE direct conjugate A cell surface stain was performed on HT-29 cells with EpCAM (AUA1) antibody NB600-1182PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Flow-Cytometry-NB600-1182-img0008.jpg)
![Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of A431 lysate (A) and Hek293 lysate (B) using EpCAM/CD326 antibody (AUA1) at 2 ug/ml. Non-reduced lysate was used in each lane.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Western-Blot-NB600-1182-img0001.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using EpCAM/CD326 antibody (blue) and matched Isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Flow-Cytometry-NB600-1182-img0007.jpg)
![Western Blot: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - Analysis of EpCAM in HT-29 lysate in the (A) absence and (B) presence of immunizing peptide using EpCAM antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-60N5D8-Western-Blot-NBP2-22381-img0013.jpg "Western Blot: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - Analysis of EpCAM in HT-29 lysate in the (A) absence and (B) presence of immunizing peptide using EpCAM antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - FFPE human colon stained with EpCAM antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-60N5D8-Immunohistochemistry-Paraffin-NBP2-22381-img0012.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - Analysis of HT-29 cells using EpCAM antibody at 0.2 ug/10^6 cells. this antibody was used for this test. Top picture shows no cell surface staining seen, bottom shows intracellular staining of EpCAM (red represents EpCAM antibody and green represents isotype control).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-60N5D8-Flow-Cytometry-NBP2-22381-img0011.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643] - Human Breast Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/826).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-826-Immunohistochemistry-Paraffin-NBP2-44643-img0003.jpg "Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643] - Human Breast Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/826).")
![Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643] - Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Monoclonal Antibody (EGP40/826) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-826-Immunofluorescence-NBP2-44643-img0001.jpg)
![Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643] - Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG1) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-826-Immunofluorescence-NBP2-44643-img0002.jpg)
![SDS-Page: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643]](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-826-SDS-Page-NBP2-44643-img0004.jpg)