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![Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NB100-58833-img0002.jpg "Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: eIF4E Antibody [NB100-58833] - Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody NB100-58833 used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by another rabbit anti-eIF4E antibody. For blotting immunoprecipitated eIF4E, NB100-58833 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunoprecipitation-NB100-58833-img0003.jpg)
![Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21524.](http://images.novusbio.com/fullsize/nbp3-21524_rabbit-eif4e-p-ser-209-mab-sr1401-5720231754887.jpg "Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21524.")
![Western Blot: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Western blot analysis using NBP3-21524 in (1) HEK293 cell lysate; (2) Mouse spleen lysate.](http://images.novusbio.com/fullsize/nbp3-21524_rabbit-eif4e-p-ser-209-mab-sr1401-6720239533793.jpg)
![Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP2-57098-img0002.jpg "Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody [NBP2-57098] - Staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57098-img0001.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21317_rabbit-eif4e-pab-4520231553437.jpg)
![Western Blot: eIF4E Antibody (7A7P2) [NBP3-15629] - Western blot analysis of extracts of various cell lines, using eIF4E antibody (NBP3-15629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/eIF4E-Antibody-7A7P2-Western-Blot-NBP3-15629-img0001.jpg "Western Blot: eIF4E Antibody (7A7P2) [NBP3-15629] - Western blot analysis of extracts of various cell lines, using eIF4E antibody (NBP3-15629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunoprecipitation: eIF4E Antibody (7A7P2) [NBP3-15629] - Immunoprecipitation analysis of 600ug extracts of Mouse testis using 3ug eIF4E antibody (NBP3-15629). Western blot was performed from the immunoprecipitate using eIF4E (NBP3-15629) at a dilition of 1:500.](http://images.novusbio.com/fullsize/eIF4E-Antibody-7A7P2-Immunoprecipitation-NBP3-15629-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF4E Antibody [NBP2-98867] - Immunochemical staining of human eIF4E in human hepatoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunohistochemistry-Paraffin-NBP2-98867-img0002.jpg "Immunohistochemistry-Paraffin: eIF4E Antibody [NBP2-98867] - Immunochemical staining of human eIF4E in human hepatoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: eIF4E Antibody [NBP2-98867] - Immunochemical staining of human eIF4E in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunohistochemistry-Paraffin-NBP2-98867-img0001.jpg)
![Western Blot: eIF4E Antibody [NBP2-98868] - Anti-eIF4E rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP2-98868-img0001.jpg "Western Blot: eIF4E Antibody [NBP2-98868] - Anti-eIF4E rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa (We are unsure as to the identity of these extra bands).")
![SDS-Page: Recombinant Mouse eIF4E Protein [NBP2-59571] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Mouse-eIF4E-Protein-SDS-Page-NBP2-59571-img0001.jpg "SDS-Page: Recombinant Mouse eIF4E Protein [NBP2-59571] - 15% SDS-PAGE (3ug)")