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![Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/VPS34-Antibody-Simple-Western-NB110-87320-img0006.jpg "Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: VPS34 Antibody [NB110-87320] - ATG7 depleted SKBR3 cells show increased sensitivity to ATRA and the RARalpha agonist AM580. RARalpha , ATG5 and VPS34 Western blot analysis of control and the respective SKBR3 knockdown cells. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VPS34-Antibody-Knockdown-Validated-NB110-87320-img0007.jpg)
![Western Blot: VPS34 Antibody [NB110-87320] - HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/VPS34-Antibody-Western-Blot-NB110-87320-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody [NB110-87320] - VPS34 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunocytochemistry-Immunofluorescence-NB110-87320-img0005.jpg)
![Western Blot: VPS34 Antibody [NB110-87320] - Celastrol regulates autophagy- & mitophagy-related gene expressions in neurons. SH-SY5Y cells treated with 0.1–1 μM celastrol for 4 h. (A) Real-time quantitative PCR results show that 1 μM celastrol treatment for 4 h enhanced mRNA expressions of phagophore induction genes Beclin 1 & Ambra1, phagophore elongation genes Atg7 & Atg12, & vacuole formation genes LC3A & Atg4B. (B) Western blot results of SH-SY5Y treated with 100–500 nM celastrol show celastrol (500 nM) increased in protein expressions of Beclin 1 & Vps34 (phagophore induction), Atg7 (phagophore elongation), LC3-II (vacuole formation), DJ-1, & PINK1 (mitophagy). Celastrol (500 nM) suppressed LRRK2 expression. (C) MPTP (10 mg/kg/day for 3 days, i.p.) but not celastrol (3 mg/kg/day for 3 days, i.p.) caused dopaminergic nerve terminal degeneration (tyrosine hydroxylase↓) & mitophagy inactivation (DJ-1↓ & PINK1↓) in the striatum of mice as compared to saline control. Celastrol cotreatment with MPTP reversed it as compared to MPTP. Data are given as the mean ± SEM (n = 3–5). p-value was determined using the Kruskal–Wallis test followed by Dunn’s multiple comparison post hoc test. * p < 0.05 compared with controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-87320_rabbit-polyclonal-vps34-antibody-310202416205164.jpg)
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - VPS34 antibody [HL2305] detects VPS34 protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS34 stained by VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249413371.jpg "Immunocytochemistry/Immunofluorescence: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - VPS34 antibody [HL2305] detects VPS34 protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS34 stained by VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249425434.jpg)
![Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249415921.jpg)
![Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249425433.jpg)
![Immunohistochemistry-Paraffin: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - VPS34 antibody [HL2305] detects VPS34 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VPS34 stained by VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249342719.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-212024939136.jpg)
![Western Blot: VPS34 Antibody [NBP1-86862] - Analysis in control (vector only transfected HEK293T lysate) and PIK3C3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/VPS34-Antibody-Western-Blot-NBP1-86862-img0006.jpg "Western Blot: VPS34 Antibody [NBP1-86862] - Analysis in control (vector only transfected HEK293T lysate) and PIK3C3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunohistochemistry-Paraffin-NBP1-86862-img0008.jpg)
![Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunohistochemistry-Paraffin-NBP1-86862-img0002.jpg)
![Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunohistochemistry-Paraffin-NBP1-86862-img0007.jpg)
![Western Blot: VPS34 Antibody [NBP2-94087] - Western blot analysis of extracts of various cell lines, using VPS34 antibody (NBP2-94087) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/VPS34 Antibody-Western Blot-NBP2-94087-img0002.jpg "Western Blot: VPS34 Antibody [NBP2-94087] - Western blot analysis of extracts of various cell lines, using VPS34 antibody (NBP2-94087) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody [NBP2-94087] - Immunofluorescence analysis of NIH/3T3 cells using VPS34 Rabbit pAb (NBP2-94087) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94087-img0001.jpg)
![Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Western-Blot-NBP3-15441-img0002.jpg "Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunofluorescence analysis of NIH-3T3 cells using VPS34 Rabbit mAb (NBP3-15441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Immunocytochemistry-Immunofluorescence-NBP3-15441-img0001.jpg)
![Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug VPS34 antibody (NBP3-15441). Western blot was performed from the immunoprecipitate using VPS34 antibody (NBP3-15441) at a dilition of 1:500.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Immunoprecipitation-NBP3-15441-img0003.jpg)
![Western Blot: VPS34 Antibody [NBP2-94870] - Analysis of extracts of various cell lines, using VPS34 at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/VPS34-Antibody-Western-Blot-NBP2-94870-img0001.jpg "Western Blot: VPS34 Antibody [NBP2-94870] - Analysis of extracts of various cell lines, using VPS34 at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody [NBP2-94870] - Immunofluorescence analysis of PC-12 cells using VPS34 antibody (NBP2-94870) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94870-img0002.jpg)
![Western Blot: VPS34 Antibody [NBP2-95117] - Analysis of extracts of various cell lines, using VPS34 . Exposure time: 90s.](http://images.novusbio.com/fullsize/VPS34-Antibody-Western-Blot-NBP2-95117-img0002.jpg "Western Blot: VPS34 Antibody [NBP2-95117] - Analysis of extracts of various cell lines, using VPS34 . Exposure time: 90s.")