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NB300-232

SCP3/SYCP3 Antibody - BSA Free

(2 Reviews)

(78 Publications)

0.1 ml

NB300-232

Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a specific signal of SCP3 in the spermatogonial cells and the spermatocytes. The signal was strongest in the nuclei of spermatogonial cells.

Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. Immunolabeling of the oocyte chromosomes with anti-SYCP3 antibody (red) and Hoechst 33342 (blue). All experiments were repeated at least three times independently. (* P<0.05; ** P<0.01) Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2017.350), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes showing that all telomeres are associated with SUN1. Anti-SUN1 staining in co-localisation with SYCP3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003261), licensed under a CC-BY license.

Western Blot: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - Analysis of SCP3 in mouse testis protein.

Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - Analysis of SCP3 in human testis using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - SCP3 labeled in mouse pachytene preparation (red), using NB300-232 SCP3 antibody. CDK2 staining, near teleomeres, is also present (green).

Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment.. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes showing that all telomeres are associated with SUN1. TeloFISH in co-localisation with SYCP3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003261), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC-P analysis of formalin fixed paraffin embedded tissue section of mouse testes using SCP3 antibody at 1:200 dilution. Specific staining may be seen in the spermatogonial cells and the primary spermatocytes.

CYP51 regulates the expression of the meiosis-specific cohesin subunits REC8 and STAG3. (A,B) RS21745 treatment decreased REC8 and STAG3 expression. Ovaries at 14.5 dpc were cultured with 10 uM RS21745 for 3 days in vitro. (A) Expression of the cohesin subunits following RS21745 treatment by qRT-PCR. All qRT-PCR values were normalized to beta-actin and were expressed as a relative ratio to the control; the means+/-s.e.m. of 3 values are shown. (B) Expression of REC8 and STAG3 following RS21745 treatment by western blotting GAPDH was used as internal reference. (C-F) Inhibition of CYP51 disturbed the distribution of both REC8 and STAG3 on the chromosomes. (C,E) In the control groups, REC8 and STAG3 expression was observed at high levels in zygotene oocytes. In the CYP51 inhibition groups, the cohesin signals were absent in zygotene cells. (D,F) The percentage of REC8-positive or STAG3-positive oocytes at the zygotene stage was recorded. Unidentified germ cells were not included in the analyses. Scale bars: 10 um. The data are presented as the means+/-s.e.m. of 3-9 ovaries per group. Asterisk (*) denotes a statistically significant difference between the control and the treatment groups. ***P<0.001 (t-test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30420384), licensed under a CC-BY licence.

Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. (a) Immunolabeling of the oocyte chromosomes with anti-SYCP3 antibody (red) and Hoechst 33342 (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28771232), licensed under a CC-BY licence.

Dose-dependent decrease or increase of the number of meiotic germ cells (SCP3, MLH1) and of gammaH2AX positive cell, respectively, in nicotine treated fetal ovaries cultured for 4 days. (A) Representative IF images of ovarian tissue sections for SCP3. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30001218), licensed under a CC-BY licence.

Induction of meiosis is compromised in the cortical germ cells of D17 embryos subject to oestrogen level alterations. (B) Sections from the D17 (HH43) left gonad shown in A immunostained for gammaH2AX (green) and SYCP3 (red). ZW-WT, ZW wild type; ZW-Fa, ZW treated with fadrozole from D7-7.5 (HH31); ZW-E2, ZW treated with beta-oestradiol at D7-7.5; ZZ-E2, ZZ treated with beta-oestradiol at D7-7.5; ZW-Fa(sr), ZW, partially sex-reversed gonad, treated with fadrozole at D4; ZZ-E2(sr), ZZ, partially sex-reversed gonad, treated with beta-oestradiol at D4. All gonadal models have a cortical domain containing germ cells. In ZW-Fa ovary and ZW-E2 ovary, most cortical germ cells express SYCP3 like the ZW-WT control. In ZW-Fa(sr) ovotestis, very few germ cells express gammaH2AX and SYCP3 (orange dotted circled areas). In ZZ-E2(sr) ovotestis and ZZ-E2 testis overlain by a cortex, some cortical germ cells express gammaH2AX but none expresses SYCP3. White dotted line highlights the cortical domain borders. See Fig. S4 for the medullary structure of the ZZ-E2, ZW-Fa(sr) and ZZ-E2(sr) models. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001442), licensed under a CC-BY licence.

NSCs Reduce Succinate Levels and Reprogram the Metabolism of Type 1 Pro-inflammatory Mφ toward Oxidative Phosphorylation In Vitro(A) Experimental setup for in vitro MφLPS co-cultures with iNSCs/NSCs.(B and C) Gene expression microarrays of MφLPS-iNSCs/NSCs. (B) Venn diagram of differentially expressed genes (adjusted p value < 0.1). (C) Heatmap of genes differentially expressed (adjusted p value < 0.1) in MφLPS-iNSCs or MφLPS-NSCs.(D and E) qRT-PCR independent validation of differentially expressed inflammatory genes as in (C). (D) Expression of genes related to type 1 inflammatory (E) and anti-inflammatory Mφ phenotypes relative to Actb. Data are mean fold change (±SEM) versus MφLPS from n ≥ 3 independent replicates per condition.(F) qRT-PCR of BV2LPS-iNSCs/NSCs (±SEM) from n ≥ 3 independent experiments per condition. BV2 and BV2LPS are shown as controls.(G and H) Extracellular flux (XF) assay of the oxygen consumption rate (OCR) (G) and extracellular acidification rate (ECAR) (H) in MφLPS-iNSCs/NSCs. Data were normalized on total protein content and are expressed as mean values (±SEM) from n ≥ 3 independent experiments per condition.(I and J) Levels of significantly changed extracellular (EXTRA_Metab, I) and intracellular (INTRA_Metab, J) metabolites in MφLPS versus Mφ at 25 hr. Data are mean a.u. (±SEM) from n ≥ 2 independent experiments per condition.(K and L) Hif-1 alpha (K), PKM2 (K), and IL-1 beta (L) expression levels relative to beta -actin. Data are mean fold change versus MφLPS (±SEM) from n ≥ 3 independent experiments per condition.∗p ≤ 0.05 and ∗∗p ≤ 0.01 versus MφLPS. See also Tables S2 and S3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29478844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Lp(a) upregulates GPx1 and Prdx6 expression in human HepG2 cells. HepG2 cells were treated with 5 μg/mL of Lp(a) or LDL for 6 hours at 37°C. Western blots of cell lysates were performed with an anti-Gpx1 antibody (a), an anti-Prdx6 antibody (b), an anti-Sod1 antibody (c), and an anti-Park7 antibody (d) using an anti-actin antibody as a loading control. Representative blots are shown. Protein levels were normalized against actin and expressed relative to that of untreated cells. Results are expressed as mean ± SEM for pooled triplicate incubations run in quadruplicate. ∗P<0.05, relative to untreated HepG2 cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30596094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Metformin-mediated AMPK activation is involved in suppression of TGF-beta -induced NOX4 expression in LF. a WB using anti-NOX4, and anti-beta -actin of cell lysates from control (lane 1, 2) and metformin (lane 3, 4) treated LF. Metformin treatment was started 1 h before TGF-beta (2 ng/ml) stimulation and protein samples were collected after 24 h treatment with TGF-beta . Lower panel is the average (±SEM) taken from three independent experiments shown as relative expression. Open bar is control and filled bar is metformin treated. *p < 0.05. b Left panel: LF were treated with TGF-beta and mRNA samples were collected at indicated time points (n = 9). *p < 0.05. Right panel: LF were treated with TGF-beta in the presence or absence of metformin (10 mM) and mRNA samples were collected after 12 h treatment with TGF-beta (n = 6). Open bar is control and filled bar is metformin treated. Real time-PCR was performed using primers to NOX4 or beta -actin, as a control. NOX4 expression was normalized to beta -actin. Shown is the fold increase (±SEM) relative to control treated cells. *p < 0.05. c WB using anti-NOX4, anti-type I collagen, anti-alpha -smooth muscle actin (SMA) and anti-beta -actin of cell lysates from control siRNA (lane 1, 2) and NOX4 siRNA (lane 3, 4) transfected LF. TGF-beta (2 ng/ml) treatment was started 48 h post transfection. Protein samples were collected after 24 h treatment with TGF-beta . In the right panels are the average (±SEM) taken from four independent experiments shown as relative expression. Open bar is control and filled bar is TGF-beta treated. *p < 0.05. d WB using anti-phospho-AMPK, anti-NOX4, anti-type I collagen, anti-alpha SMA, and anti-beta -actin of cell lysates from control siRNA (lane 1, 2, 3, 4) and AMPK siRNA (lane 5, 6) transfected LF. Metformin treatment was started 48 h post transfection and 1 h before TGF-beta (2 ng/ml) stimulation, and protein samples were collected after 24 h treatment with TGF-beta . In the right panels are the average (±SEM) taken from five independent experiments shown as relative expression. Open bar is control and filled bar is TGF-beta treated. *p < 0.05 Image collected and cropped by CiteAb from the following open publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-016-0420-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Fe, Pa

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Fe, Pa

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-232AF350
Alexa Fluor 405	NB300-232AF405
Alexa Fluor 488	NB300-232AF488
Alexa Fluor 532	NB300-232AF532
Alexa Fluor 594	NB300-232AF594
Alexa Fluor 647	NB300-232AF647
Alexa Fluor 700	NB300-232AF700
Alexa Fluor 750	NB300-232AF750
Allophycocyanin	NB300-232APC
Biotin	NB300-232B
DyLight 350	NB300-232UV
DyLight 405	NB300-232V
DyLight 488	NB300-232G
DyLight 550	NB300-232R
DyLight 594	NB300-232DL594
DyLight 650	NB300-232C
DyLight 680	NB300-232FR
DyLight 755	NB300-232IR
FITC	NB300-232F
HRP	NB300-232H
Janelia Fluor 549	NB300-232JF549
Janelia Fluor 646	NB300-232JF646
PE	NB300-232PE
PerCP	NB300-232PCP
mFluor Violet 450 SE	NB300-232MFV450
mFluor Violet 500 SE	NB300-232MFV500
mFluor Violet 610 SE	NB300-232MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-232SS

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NBP2-78133

SCP3/SYCP3 Antibody - BSA Free

0.1 mg

NBP2-78133

Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NBP2-78133] - IHC analysis of a FFPE tissue section of the mouse testis using 1:200 dilution of SCP3 antibody (NBP2-78133). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated strong staining associated with chromatin in meiotic cells.

Reactivity:

Hu, Mu, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Bv, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-78133AF350
Alexa Fluor 405	NBP2-78133AF405
Alexa Fluor 488	NBP2-78133AF488
Alexa Fluor 532	NBP2-78133AF532
Alexa Fluor 594	NBP2-78133AF594
Alexa Fluor 647	NBP2-78133AF647
Alexa Fluor 700	NBP2-78133AF700
Alexa Fluor 750	NBP2-78133AF750
Allophycocyanin	NBP2-78133APC
Biotin	NBP2-78133B
DyLight 350	NBP2-78133UV
DyLight 405	NBP2-78133V
DyLight 488	NBP2-78133G
DyLight 550	NBP2-78133R
DyLight 594	NBP2-78133DL594
DyLight 650	NBP2-78133C
DyLight 680	NBP2-78133FR
DyLight 755	NBP2-78133IR
FITC	NBP2-78133F
HRP	NBP2-78133H
Janelia Fluor 549	NBP2-78133JF549
Janelia Fluor 646	NBP2-78133JF646
PE	NBP2-78133PE
PerCP	NBP2-78133PCP
mFluor Violet 450 SE	NBP2-78133MFV450
mFluor Violet 500 SE	NBP2-78133MFV500
mFluor Violet 610 SE	NBP2-78133MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-78133SS

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NB300-232G

SCP3/SYCP3 Antibody [DyLight 488]

0.1 ml

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Fe, Pa

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Fe, Pa

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

DyLight 488

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-232AF350
Alexa Fluor 405	NB300-232AF405
Alexa Fluor 488	NB300-232AF488
Alexa Fluor 532	NB300-232AF532
Alexa Fluor 594	NB300-232AF594
Alexa Fluor 647	NB300-232AF647
Alexa Fluor 700	NB300-232AF700
Alexa Fluor 750	NB300-232AF750
Biotin	NB300-232B
DyLight 350	NB300-232UV
DyLight 405	NB300-232V
DyLight 550	NB300-232R
DyLight 594	NB300-232DL594
DyLight 650	NB300-232C
DyLight 680	NB300-232FR
DyLight 755	NB300-232IR
FITC	NB300-232F
HRP	NB300-232H
Janelia Fluor 549	NB300-232JF549
Janelia Fluor 646	NB300-232JF646

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-232SS
BSA Free	NB300-232

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(select up to 3 products from this page to compare)

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