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![Western Blot: Otubain-1 Antibody [NBP1-83670] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-83670-img0019.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Otubain-1 Antibody [NBP1-83670] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Otubain-1 Antibody [NBP1-83670] - Staining of human Fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Immunohistochemistry-Paraffin-NBP1-83670-img0020.jpg)
![Western Blot: Otubain-1 Antibody [NBP1-83670] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-83670-img0017.jpg)
![Western Blot: Otubain-1 Antibody [NBP1-49934] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-49934-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Otubain-1 Antibody [NBP1-49934] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![Western Blot: Otubain-1 Antibody [NBP3-13214] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody (NBP3-13214) diluted at 1:1000.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP3-13214-img0001.jpg "Western Blot: Otubain-1 Antibody [NBP3-13214] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody (NBP3-13214) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody [NBP3-13214] - Otubain-1 antibody detects Otubain-1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Otubain-1 stained by Otubain-1 antibody (NBP3-13214) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13214-img0002.jpg)
![Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-GT611-Western-Blot-NBP3-13627-img0001.jpg "Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody (GT611) [NBP3-13627] - Otubain-1 antibody [GT611] detects Otubain-1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Otubain-1 stained by Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-GT611-Immunocytochemistry-Immunofluorescence-NBP3-13627-img0002.jpg)
![Western Blot: Otubain-1 Antibody [NBP1-20957] - Staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-20957-img0002.jpg "Western Blot: Otubain-1 Antibody [NBP1-20957] - Staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Otubain-1 Antibody [NBP1-49935] - OTUB1 Antibody [NBP1-49935] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-49935-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Otubain-1 Antibody [NBP1-49935] - OTUB1 Antibody [NBP1-49935] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: Otubain-1 Antibody [NBP2-99440] - Anti-Otubain-1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP2-99440-img0002.jpg "Western Blot: Otubain-1 Antibody [NBP2-99440] - Anti-Otubain-1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa")