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NB100-2331

LC3A Antibody - BSA Free

(22 Reviews)

(296 Publications)

0.1 ml

NB100-2331

Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review.

Immunohistochemistry: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Staining in mouse meniscus and cartilage. Image from verified customer review.

Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Autophagy mobilized during differentiation of immortalized human mesenchymal stem cells (ihMSCs). Cultured ihMSCs were stimulated to undergo osteogenesis to form osteoblasts for 30 days at 21% and 4% oxygen. LC3I and LC3II levels were assessed via immunoblot every 10 days and normalized to beta-actin to derive average LC3II band densities. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25523618/) licensed under a CC-BY license.

Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Autophagy mobilized during differentiation of immortalized human mesenchymal stem cells (ihMSCs). Cultured ihMSCs were stimulated to undergo osteogenesis to form osteoblasts for 30 days at 21% and 4% oxygen. LC3I and LC3II levels were assessed via immunoblot every 10 days and normalized to beta-actin to derive average LC3II band densities. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license.

Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows Analysis in human cell lysates.

Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows analysis of heart tissue lysates from mice which were subjected or not to 48 hours of starvation. The signal was developed using ECL method and this LC3 antibody was found to detect both forms of LC3, i.e. LC3A and LC3B. As expected, the levels of LC3B form were higher in the heart tissue lysates from starved mice.

Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Detection of HRP conjugated autophagic LC3 in mouse ES cell lysate. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.

Immunocytochemistry/Immunofluorescence: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows an analysis in HeLa cells using anti-LC3 antibody (red). Nuclei were counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Left panel shows untreated HeLa cells. Right panel shows HeLa cells that were treated with 50 uM CQ overnight. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-LC3A antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license.

Immunohistochemistry: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Analysis in mouse renal tissue. Image from verifed customer review.

Simple Western: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Image shows a specific band for LC3 in 0.5 mg/mL of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Identification of an autophagy enhancer small-molecule (MSL) inducing nuclear translocation of TFEB. g) WT and Tfeb knockout HeLa cells were treated with MSL in the presence or absence of bafilomycin A1 (BafA), and cell extract was subjected to Western blot analysis using the indicated antibodies. Numbers below LC3 immunoblot bands indicate fold changes of LC3-II normalized to beta-actin bands. All data in this figure are the means +/- s.e.m. from >=3 independent experiments performed in triplicate (scale bar, 20 um). **P 0.05. Shown are the mean ±SEM for three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Truncated PPM1D impairs DNA damage response in mouse thymus. Expression of PPM1D mRNA was analyzed by RT-qPCR in the thymi of Ppm1d+/+, Ppm1dT/+ and Ppm1dT/T mice and was normalized to GAPDH (n = 3) (A). Thymi from mice of indicated genotypes were lysed and proteins were separated by SDS-PAGE. Samples were probed with antibody against PPM1D and importin-beta as a loading control. The empty and full arrowheads indicate the position of the full-length and the C-terminally truncated PPM1D, respectively. (B). Cells from thymi from Ppm1d+/+ and Ppm1dT/+ mice were analyzed by flow cytometry. Plotted are the counts of the indicated populations as follows: double-negative T-cells (DN and DN1, DN2, DN3, DN4), double-positive T-cells (DP), CD8-positive T-cells (CD8+) and CD4-positive T-cells (CD4+) (n = 3) (C). The median size of the thymus was determined in Ppm1d +/+ (n = 11) and Ppm1dT/+ (n = 12) mice (D). A scheme of the experimental setup in F-I. Mice were exposed or not to a low dose of IR (3 Gy), sacrificed after 6 h and thymi and lymph nodes were collected (E). Proteins isolated from thymi from mice of indicated genotypes exposed to mock or to IR were probed with the indicated antibodies by immunoblotting (F). Proteins isolated from inguinal lymph nodes from mice of indicated genotypes exposed to mock or to IR were probed with the indicated antibodies by immunoblotting (G). RNA isolated from thymi from mice in E was analyzed by RT-qPCR. The expression of CDKN1Ap21 mRNA was normalized to GAPDH. Statistical significance was evaluated by two-tailed t-test, error bars indicate SD, n = 5 (H). RNA isolated from thymi from mice in D was analyzed by RT-qPCR. The expression of PUMA mRNA was normalized to GAPDH. Statistical significance was evaluated by two-tailed t-test, error bars indicate SD, n = 5. * p <0.05; *** p < 0.0005; **** p < 0.0001 (I). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32927737), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

KDM1A inhibition impairs cell proliferation and migration and induces apoptosis in human medulloblastoma cell lines. a Bars represent KDM1A expression measured using real-time RT-PCR and normalized to the geometric mean of GAPDH, UBC and HPRT expression in DAOY and ONS-76 cell lines 72 h after KDM1A knockdown or mock transfection. ***p < 0.0001 b Knockdown of KDM1A protein was confirmed by western blotting of whole-cell lysates from DAOY and ONS-76 cells. beta -actin served as loading control. c The DAOY and ONS-76 medulloblastoma cell lines were transfected with siRNA directed against KDMA1, and cell viability was measured using the MTT assay. Extinction relative to mock-transfected cultures at 72 h is shown. ***p < 0.0001 d Proliferation of DAOY and ONS-76 cells following mock transfection or transfection with siRNA directed against KDM1A was assessed by BrdU ELISA. Bars show extinction relative to mock-transfected cultures at 72 h. ***p < 0.0001 e Apoptosis in DAOY and ONS-76 cells was measured by Cell Death Detection ELISA™ 72 h after transfection with either siRNA directed against KDMA1 or mock transfection. Extinction is relative to mock-transfected cultures. ***p < 0.0001, *p < 0.05 f Migratory activity was assessed for the ONS-76 cell line 48 h after transfection with either siRNA directed against KDM1A or mock transfection in Boyden chamber assays. Representative images of DAPI-stained mock-transfected control cells (ONS-76 ctrl) and KDM1A-knockdown cells (ONS-76 siKDM1A) invading the membrane (scale bars = 100 μm). g Statistical analysis of results from Boyden chamber assays 24 h after DAOY and ONS-76 cells, either transfected with siRNA directed against KDM1A or mock-transfected, were plated in the upper chamber. Bars display quantity of cells per mm square which migrated through the membrane. **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24252778), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of ER stress markers in mouse and human skeletal muscle(a) TA muscle lysates from high fat fed mice were used to determine the expression of ER stress markers by Western blot analysis (n=6). (b-g) Western blot analysis was done to measure proteins involved in ER stress in vastus lateralis muscle from lean, obese, or diabetic subjects (n=6-13). Data are the means ± S.E.M. * indicates p<0.05 and ** indicates p<0.01 vs. chow or lean controls, using Student’s t-test (a) and One way ANOVA. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/ncomms2851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The effect of ATG5 and ATG7 on autophagy, ER stress, apoptosis and cell cycle depends on PERK. a The ER stress proteins were analysed by western blotting in the GSK2606414 treated chondrocytes. b The levels of ER stress proteins were normalized to beta -actin. c Determination of autophagy and apoptosis proteins expression by western blotting in the GSK2606414 treated chondrocytes. d The levels of related proteins were normalized to beta -actin. e FCM analysis was used to calculate the percentage of apoptotic cells at the time point of 24 h. The apoptosis rate were increased when combined treated with GSK2606414. Experiments were repeated 3 times, Representative images are shown. f Analysis of cell apoptosis. Data come from 3 independent experiments. g FCM analysis indicated that the S phase percentage were decreased compared to that of the control groups when combine treated with GSK2606414 in C28I2 cells. Experiments were repeated 3 times, Representative images are shown. h Percentage of cells at each phase in different groups. *P < 0.05, **P < 0.01 compared with the controls. Values are means ± SD n = 3). (1:Ad-GFP, 2:Ad-ATG5 + Ad-ATG7, 3:Ad-ATG5 + Ad-ATG7 + DMSO, 4:Ad-ATG5 + Ad-ATG7+ GSK2606414) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31060556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Am, Ca, Fi, Pl, Ze, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Ca, Fi, Pl, Ze, Bv

Applications:

, , , , , , , , , , ,

Applications: , , , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2331AF350
Alexa Fluor 405	NB100-2331AF405
Alexa Fluor 488	NB100-2331AF488
Alexa Fluor 532	NB100-2331AF532
Alexa Fluor 594	NB100-2331AF594
Alexa Fluor 647	NB100-2331AF647
Alexa Fluor 700	NB100-2331AF700
Alexa Fluor 750	NB100-2331AF750
Allophycocyanin	NB100-2331APC
Biotin	NB100-2331B
DyLight 350	NB100-2331UV
DyLight 405	NB100-2331V
DyLight 488	NB100-2331G
DyLight 550	NB100-2331R
DyLight 594	NB100-2331DL594
DyLight 650	NB100-2331C
DyLight 680	NB100-2331FR
DyLight 755	NB100-2331IR
FITC	NB100-2331F
HRP	NB100-2331H
Janelia Fluor 549	NB100-2331JF549
Janelia Fluor 646	NB100-2331JF646
PE	NB100-2331PE
PerCP	NB100-2331PCP
mFluor Violet 450 SE	NB100-2331MFV450
mFluor Violet 500 SE	NB100-2331MFV500
mFluor Violet 610 SE	NB100-2331MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2331SS

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NBP1-78964

LC3A Antibody - BSA Free

(1 Review)

0.1 ml

NBP1-78964

Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.

Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-78964] - IF Confocal analysis of A549 cells using LC3I antibody (NBP1-78964, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry: LC3A Antibody [NBP1-78964] - Analysis of LC3I in mouse testis using DAB with hematoxylin counterstain.

Reactivity:

Hu, Mu, Rt, Po, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78964AF350
Alexa Fluor 405	NBP1-78964AF405
Alexa Fluor 488	NBP1-78964AF488
Alexa Fluor 532	NBP1-78964AF532
Alexa Fluor 594	NBP1-78964AF594
Alexa Fluor 647	NBP1-78964AF647
Alexa Fluor 700	NBP1-78964AF700
Alexa Fluor 750	NBP1-78964AF750
Allophycocyanin	NBP1-78964APC
Biotin	NBP1-78964B
DyLight 350	NBP1-78964UV
DyLight 405	NBP1-78964V
DyLight 488	NBP1-78964G
DyLight 550	NBP1-78964R
DyLight 594	NBP1-78964DL594
DyLight 650	NBP1-78964C
DyLight 680	NBP1-78964FR
DyLight 755	NBP1-78964IR
FITC	NBP1-78964F
HRP	NBP1-78964H
Janelia Fluor 549	NBP1-78964JF549
Janelia Fluor 646	NBP1-78964JF646
PE	NBP1-78964PE
PerCP	NBP1-78964PCP
mFluor Violet 450 SE	NBP1-78964MFV450
mFluor Violet 500 SE	NBP1-78964MFV500
mFluor Violet 610 SE	NBP1-78964MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78964SS

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NBP2-75924

LC3A Antibody (2312D) - BSA Free

0.1 mg

NBP2-75924

Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunohistochemistry-Paraffin: LC3A Antibody (2312D) [NBP2-75924] - This LC3A antibody was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody (Catalog # NBP2-75924) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-75924AF350
Alexa Fluor 405	NBP2-75924AF405
Alexa Fluor 488	NBP2-75924AF488
Alexa Fluor 532	NBP2-75924AF532
Alexa Fluor 594	NBP2-75924AF594
Alexa Fluor 647	NBP2-75924AF647
Alexa Fluor 700	NBP2-75924AF700
Alexa Fluor 750	NBP2-75924AF750
Allophycocyanin	NBP2-75924APC
Biotin	NBP2-75924B
DyLight 350	NBP2-75924UV
DyLight 405	NBP2-75924V
DyLight 488	NBP2-75924G
DyLight 550	NBP2-75924R
DyLight 594	NBP2-75924DL594
DyLight 650	NBP2-75924C
DyLight 680	NBP2-75924FR
DyLight 755	NBP2-75924IR
FITC	NBP2-75924F
HRP	NBP2-75924H
Janelia Fluor 549	NBP2-75924JF549
Janelia Fluor 646	NBP2-75924JF646
PE	NBP2-75924PE
PerCP	NBP2-75924PCP
mFluor Violet 450 SE	NBP2-75924MFV450
mFluor Violet 500 SE	NBP2-75924MFV500
mFluor Violet 610 SE	NBP2-75924MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-75924SS
Azide and BSA Free	NBP2-80828

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NBP1-78964MFV610

LC3A Antibody [mFluor Violet 610 SE]

0.1 ml

Reactivity:

Hu, Mu, Rt, Po, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

mFluor Violet 610 SE

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78964AF350
Alexa Fluor 405	NBP1-78964AF405
Alexa Fluor 488	NBP1-78964AF488
Alexa Fluor 532	NBP1-78964AF532
Alexa Fluor 594	NBP1-78964AF594
Alexa Fluor 647	NBP1-78964AF647
Alexa Fluor 700	NBP1-78964AF700
Alexa Fluor 750	NBP1-78964AF750
Allophycocyanin	NBP1-78964APC
Biotin	NBP1-78964B
DyLight 350	NBP1-78964UV
DyLight 405	NBP1-78964V
DyLight 488	NBP1-78964G
DyLight 550	NBP1-78964R
DyLight 594	NBP1-78964DL594
DyLight 650	NBP1-78964C
DyLight 680	NBP1-78964FR
DyLight 755	NBP1-78964IR
FITC	NBP1-78964F
HRP	NBP1-78964H
Janelia Fluor 549	NBP1-78964JF549
Janelia Fluor 646	NBP1-78964JF646
PE	NBP1-78964PE
PerCP	NBP1-78964PCP
mFluor Violet 450 SE	NBP1-78964MFV450
mFluor Violet 500 SE	NBP1-78964MFV500

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78964SS
BSA Free	NBP1-78964

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NBP2-75924V

LC3A Antibody (2312D) [DyLight 405]

0.1 ml

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

DyLight 405

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-75924AF350
Alexa Fluor 405	NBP2-75924AF405
Alexa Fluor 488	NBP2-75924AF488
Alexa Fluor 532	NBP2-75924AF532
Alexa Fluor 594	NBP2-75924AF594
Alexa Fluor 647	NBP2-75924AF647
Alexa Fluor 700	NBP2-75924AF700
Alexa Fluor 750	NBP2-75924AF750
Allophycocyanin	NBP2-75924APC
Biotin	NBP2-75924B
DyLight 350	NBP2-75924UV
DyLight 488	NBP2-75924G
DyLight 550	NBP2-75924R
DyLight 594	NBP2-75924DL594
DyLight 650	NBP2-75924C
DyLight 680	NBP2-75924FR
DyLight 755	NBP2-75924IR
FITC	NBP2-75924F
HRP	NBP2-75924H
Janelia Fluor 549	NBP2-75924JF549
Janelia Fluor 646	NBP2-75924JF646
PE	NBP2-75924PE
PerCP	NBP2-75924PCP
mFluor Violet 450 SE	NBP2-75924MFV450
mFluor Violet 500 SE	NBP2-75924MFV500
mFluor Violet 610 SE	NBP2-75924MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-75924SS
BSA Free	NBP2-75924
Azide and BSA Free	NBP2-80828

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