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12 Results for "novus"

NBP2-15575

APP Antibody

(1 Review)

(2 Publications)

0.1 ml

NBP2-15575

Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: APP Antibody [NBP2-15575] - SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: APP protein stained by APP antibody diluted at 1:100. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575] - Rat brain. APP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: APP Antibody [NBP2-15575] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.

Western Blot: APP Antibody [NBP2-15575] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.

Western Blot: APP Antibody [NBP2-15575] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: APP Antibody [NBP2-15575] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APP protein stained by APP antibody diluted at 1:100. Blue: Hoechst 33342 staining.

ELISA: APP Antibody [NBP2-15575] - Detection of non-transfected and transfected 293T whole cell extracts using NBP2-15575 as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-APP antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Rb

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-76910

APP Antibody - BSA Free

(4 Publications)

0.1 mg

NBP1-76910

Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/Immunofluorescence: APP Antibody [NBP1-76910] - Staining of APP in mouse brain tissue with APP Antibodyat 20 ug/mL.

Immunohistochemistry-Paraffin: APP Antibody [NBP1-76910] - Staining of APP in mouse brain tissue with at 5 ug/mL.

Immunocytochemistry/Immunofluorescence: APP Antibody [NBP1-76910] - Rat Brain cells with APP antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: APP Antibody [NBP1-76910] - Rat brain using APP antibody at 2 ug/ml.

Reactivity:

Hu, Mu, Rt, Po, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Gp

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-191

Alt Catalog# IMG-191

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76910-0.025mg

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NBP1-32081

APP Antibody

0.1 ml

NBP1-32081

Western Blot: APP Antibody [NBP1-32081] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody, C-term diluted at 1:500.

Immunocytochemistry/Immunofluorescence: APP Antibody [NBP1-32081] - APP antibody, C-term detects APP protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APP protein stained by APP antibody, C-term diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: APP Antibody [NBP1-32081] - APP antibody, C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. APP antibody, C-term diluted at 1:500.

Western Blot: APP Antibody [NBP1-32081] - Sample (30 ug of whole cell lysate) A:NIH-3T3 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: APP Antibody [NBP1-32081] - Sample(30 ug of whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1500.

Immunocytochemistry/Immunofluorescence: APP Antibody [NBP1-32081] - Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: APP protein stained by beta Amyloid antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: APP Antibody [NBP1-32081] - -embedded Tonsil, plasma cells, using antibody 10 ug/ml.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-15560

APP Antibody (3A8S2)

100 ul

NBP3-15560

Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560] - Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: APP Antibody (3A8S2) [NBP3-15560] - Immunohistochemistry of paraffin-embedded mouse brain using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: APP Antibody (3A8S2) [NBP3-15560] - Immunohistochemistry of paraffin-embedded rat brain using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: APP Antibody (3A8S2) [NBP3-15560] - Immunohistochemistry of paraffin-embedded human brain using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

<b>Genetic Strategies Validation. </b>Knockout Validated: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (NBP3-15560). Western blot was performed from the immunoprecipitate using APP antibody (NBP3-15560) at a dilition of 1:1000.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-46535

APP Antibody (OTI4C9)

0.1 ml

NBP2-46535

Western Blot: APP Antibody (4C9) [NBP2-46535] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Human liver tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Human Kidney tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Human lymphoma tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Human breast tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Adenocarcinoma of Human colon tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Human lung tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Human pancreas tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: APP Antibody (4C9) [NBP2-46535] - Analysis of Adenocarcinoma of Human breast tissue.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse monoclonal

Host: Mouse monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70197AF350
Alexa Fluor 405	NBP2-70197AF405
Alexa Fluor 488	NBP2-70197AF488
Alexa Fluor 532	NBP2-70197AF532
Alexa Fluor 594	NBP2-70197AF594
Alexa Fluor 647	NBP2-70197AF647
Alexa Fluor 700	NBP2-70197AF700
Alexa Fluor 750	NBP2-70197AF750
Allophycocyanin	NBP2-70197APC
Biotin	NBP2-70197B
DyLight 350	NBP2-70197UV
DyLight 405	NBP2-70197V
DyLight 488	NBP2-70197G
DyLight 550	NBP2-70197R
DyLight 594	NBP2-70197DL594
DyLight 650	NBP2-70197C
DyLight 680	NBP2-70197FR
DyLight 755	NBP2-70197IR
FITC	NBP2-70197F
HRP	NBP2-70197H
Janelia Fluor 549	NBP2-70197JF549
Janelia Fluor 646	NBP2-70197JF646
PE	NBP2-70197PE
PerCP	NBP2-70197PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70197

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AF2508

APP [p Thr668] Antibody

(1 Publication)

100 μg

AF2508

Western blot shows lysates of L-929 mouse fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-APP/Protease Nexin II (T668) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2508), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

APP/Protease Nexin II phosphorylated at T668 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain (cortex) using Rabbit Anti-Human/Mouse Phospho-APP/Protease Nexin II (T668) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2508) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2508-SP

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NBP1-90246

APP Antibody

(1 Publication)

0.1 ml

NBP1-90246

Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding APP RNA-seq data are presented for the same tissues.

<b>Orthogonal Strategies Validation. </b>Western Blot: APP Antibody [NBP1-90246] - Analysis in control (vector only transfected HEK293T lysate) and APP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human hippocampus shows moderate granular positivity in cytoplasm in neurons.

Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human cerebellum shows moderate granular positivity in cytoplasm in Purkinje cells.

Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human cerebral cortex shows moderate granular positivity in cytoplasm in neurons.

Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB2508

APP [p Thr668] Antibody (304904)

100 μg

MAB2508

APP/Protease Nexin II was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex) using 8 µg/mL Human/Mouse Phospho-APP/Protease Nexin II (T668) Monoclonal Antibody (Catalog # MAB2508) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole for 16 hours. PVDF membrane was probed with 1 µg/mL of Human/Mouse Phospho-APP/Protease Nexin II (T668) Monoclonal Antibody (Catalog # MAB2508), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2508-SP

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NB300-308

APP Antibody

(1 Publication)

0.1 ml

NB300-308

Western Blot: APP Antibody [NB300-308]

Immunocytochemistry/Immunofluorescence: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) APP. Overlay images indicate the possible co-localization of VDR/APP on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.

Simultaneous suppression of MTH1 and MYH was efficiently achieved using a two-vector system. (a) Steps of cell line establishment are illustrated here. Top 15% of cells expressing GFP and RFP670 were sorted to improve the knockdown efficiency. Expression levels of MYH and MTH1 were analyzed after 96 h of treatment with Dox in shRNA set1 (b) and shRNA set2 (c) using qRT–PCR analysis. Data were normalized to NT-shRNA expressing cells and presented as mean±s.e.m. from three independent experiments in triplicate. To investigate the knockdown efficiency at protein level, western blot analysis was performed after 96 h of Dox treatment of cells with shRNA set1 (d) and shRNA set2 (e). Data were double normalized with GAPDH and NT-shRNA control samples and expressed as percentage. The graphs represent mean±s.e.m. from three independent experiments. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, ***P<0.001, ****P⩽0.0001, NS, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27918552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Mu, Rt, Hu, Ca, Ch, Pm

Species Glossary

Reactivity: Mu, Rt, Hu, Ca, Ch, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-04263

APP Antibody (J4H9) - BSA Free

0.1 ml

NBP1-04263

Western Blot: APP Antibody (J4H9) [NBP1-04263] - The lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse brain tissue lysate.

Immunocytochemistry/Immunofluorescence: APP Antibody (J4H9) [NBP1-04263] - Analysis of APP/Protease Nexin II in U87MG cells. The cell was stained using a dilution of (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Flow Cytometry: APP Antibody (J4H9) [NBP1-04263] - Flow analysis of APP/Protease Nexin II in 293T cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-04263AF350
Alexa Fluor 405	NBP1-04263AF405
Alexa Fluor 488	NBP1-04263AF488
Alexa Fluor 532	NBP1-04263AF532
Alexa Fluor 594	NBP1-04263AF594
Alexa Fluor 647	NBP1-04263AF647
Alexa Fluor 700	NBP1-04263AF700
Alexa Fluor 750	NBP1-04263AF750
Biotin	NBP1-04263B
DyLight 350	NBP1-04263UV
DyLight 405	NBP1-04263V
DyLight 488	NBP1-04263G
DyLight 550	NBP1-04263R
DyLight 594	NBP1-04263DL594
DyLight 650	NBP1-04263C
DyLight 680	NBP1-04263FR
DyLight 755	NBP1-04263IR
FITC	NBP1-04263F
HRP	NBP1-04263H
Janelia Fluor 549	NBP1-04263JF549
Janelia Fluor 646	NBP1-04263JF646
mFluor Violet 450 SE	NBP1-04263MFV450
mFluor Violet 500 SE	NBP1-04263MFV500
mFluor Violet 610 SE	NBP1-04263MFV610

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NBP3-26502

APP Antibody (6F10)

100 ul

NBP3-26502

Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa

Immunocytochemistry/Immunofluorescence: APP Antibody (6F10) [NBP3-26502] - Staining of Hela Cells with APP Antibody (6F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-20015

APP Antibody (S05-3E3)

100 ul

NBP3-20015

Western Blot: APP Antibody (S05-3E3) [NBP3-20015] - Western blot detection of APP in Rat Brain, 3T3, Hela cell lysates using NBP3-20015 (1:1000 diluted). Predicted band size: 87kDa. Observed band size: 100kDa.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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