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![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.](http://images.novusbio.com/fullsize2/CD4-Antibody-(W3-25)-Flow-Cytometry-NB100-64988-img0008.jpg "Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.")
![Flow (Cell Surface): CD4 Antibody (W3/25) [NB100-64988] - Rat Splenocytes were stained with NB100-64988 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alex Fluor 488.](http://images.novusbio.com/fullsize2/CD4-Antibody-(W3-25)-Flow-(Cell-Surface)-NB100-64988-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody (W3/25) [NB100-64988] - Staining of rat splenocytes with CD4 (W3/25) antibody. Live cells were incubated with CD4 antibody on ice, then fixed with 2% formaldehyde prior to secondary antibody incubation.](http://images.novusbio.com/fullsize2/CD4-Antibody-(W3-25)-Immunocytochemistry-Immunofluorescence-NB100-64988-img0007.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody (W3/25) [NB100-64988] - Staining of frozen rat spleen with Mouse anti Rat CD4.](http://images.novusbio.com/fullsize/CD4-Antibody-(W3-25)-Immunohistochemistry-Frozen-NB100-64988-img0001.jpg)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Staining of stimulated rat spleen cells.](http://images.novusbio.com/fullsize2/CD4-Antibody-(W3-25)-Flow-Cytometry-NB100-64988-img0003.jpg)
![CD4 Antibody (W3/25) [Alexa Fluor® 405] [NB100-64988AF405] - Vial of Alexa Fluor 405 conjugated antibody. Alexa Fluor 405 is optimally excited at 401 nm by the Violet laser (405 nm) and has an emission maximum of 421 nm.](http://images.novusbio.com/fullsize/nb100-64988af405_mouse-monoclonal-cd4-antibody-w3-25-alexa-fluor-405-31720239153.jpg "CD4 Antibody (W3/25) [Alexa Fluor® 405] [NB100-64988AF405] - Vial of Alexa Fluor 405 conjugated antibody. Alexa Fluor 405 is optimally excited at 401 nm by the Violet laser (405 nm) and has an emission maximum of 421 nm.")