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![Immunohistochemistry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel). Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5 min. at room temperature. Slides were blocked with normal IgG, and incubated for 1 hour with 5ug/ml MAb to BCMA (human) (Vicky-1) in 1% BSA in 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Immunohistochemistry-NBP1-97637-img0007.jpg "Immunohistochemistry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel). Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5 min. at room temperature. Slides were blocked with normal IgG, and incubated for 1 hour with 5ug/ml MAb to BCMA (human) (Vicky-1) in 1% BSA in 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.")
![Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Analysis using the PE conjugate of NBP1-97637. Staining of 10^6 U266 cells using BCMA (human), mAb (Vicky-1) (PE conjugate) at a concentration of 10ug/ml.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Flow-Cytometry-NBP1-97637-img0011.jpg)
![Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Detection of endogenous human BCMA with MAb to BCMA (human) (Vicky-1). Method: U266 cells (2x10^5) were incubated on ice for 30 min. with 0.2ug of MAb to BCMA (human) (Vicky-1) or an isotype control in 25ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide). The primary antibody was revealed with PAb to Rat IgG (R-PE) and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Flow-Cytometry-NBP1-97637-img0006.jpg)
![Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Analysis using the FITC conjugate of NBP1-97637. Staining of 10^6 Jurkat cells using BCMA (human), mAb (Vicky-1) (FITC conjugate) at a concentration of 50ug/ml.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Flow-Cytometry-NBP1-97637-img0010.jpg)
![Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [FITC] [NBP1-97655] - Analysis of 10^6 Jurkat cells stained using BCMA (human), mAb (Vicky-1) (FITC conjugate) at a concentration of 50ug/ml.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-FITC-Flow-Cytometry-NBP1-97655-img0001.jpg "Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [FITC] [NBP1-97655] - Analysis of 10^6 Jurkat cells stained using BCMA (human), mAb (Vicky-1) (FITC conjugate) at a concentration of 50ug/ml.")