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![Western Blot: Cortactin Antibody (2W5H9) [NBP3-16813] - Western blot analysis of extracts of various cell lines, using Cortactin Rabbit mAb (NBP3-16813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Western-Blot-NBP3-16813-img0007.jpg "Western Blot: Cortactin Antibody (2W5H9) [NBP3-16813] - Western blot analysis of extracts of various cell lines, using Cortactin Rabbit mAb (NBP3-16813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody (2W5H9) [NBP3-16813] - Immunofluorescence analysis of U-2 OS cells using Cortactin Rabbit mAb (NBP3-16813) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Immunocytochemistry-Immunofluorescence-NBP3-16813-img0006.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody (2W5H9) [NBP3-16813] - Immunohistochemistry of paraffin-embedded mouse testis using Cortactin Rabbit mAb (NBP3-16813) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Immunohistochemistry-Paraffin-NBP3-16813-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody (2W5H9) [NBP3-16813] - Immunofluorescence analysis of C6 cells using Cortactin Rabbit mAb (NBP3-16813) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Immunocytochemistry-Immunofluorescence-NBP3-16813-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody (2W5H9) [NBP3-16813] - Immunofluorescence analysis of NIH-3T3 cells using Cortactin Rabbit mAb (NBP3-16813) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Immunocytochemistry-Immunofluorescence-NBP3-16813-img0005.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody (2W5H9) [NBP3-16813] - Immunohistochemistry of paraffin-embedded rat testis using Cortactin Rabbit mAb (NBP3-16813) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Immunohistochemistry-Paraffin-NBP3-16813-img0001.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody (2W5H9) [NBP3-16813] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Cortactin Rabbit mAb (NBP3-16813) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Immunohistochemistry-Paraffin-NBP3-16813-img0002.jpg)
![Immunoprecipitation: Cortactin Antibody (2W5H9) [NBP3-16813] - Immunoprecipitation analysis of 600ug extracts of Mouse lung cells using 3ug Cortactin antibody (NBP3-16813). Western blot was performed from the immunoprecipitate using Cortactin antibody (NBP3-16813) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-2W5H9-Immunoprecipitation-NBP3-16813-img0008.jpg)
![Knockout Validated: Cortactin Antibody [NBP2-15972] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Cortactin Antibody [NBP2-15972] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15972] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] diluted at 1:10000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody [NBP2-15972] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15972-img0003.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15972] - Paraffin-embedded mouse intestine. Cortactin antibody [N1C1] diluted at 1:500.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunohistochemistry-Paraffin-NBP2-15972-img0005.jpg)
![Western Blot: Cortactin Antibody [NBP2-15972] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0007.jpg)
![Western Blot: Cortactin Antibody [NBP2-15972] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] diluted at 1:10000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0009.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15972] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunohistochemistry-Paraffin-NBP2-15972-img0002.jpg)
![Knockout Validated: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0006.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody [NBP2-15971] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cortactin protein stained by Cortactin antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15971-img0005.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunohistochemistry-Paraffin-NBP2-15971-img0004.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0001.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0002.jpg)
![Western Blot: Cortactin Antibody [NBP2-38826] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-38826-img0002.jpg "Western Blot: Cortactin Antibody [NBP2-38826] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue")
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody [NBP2-38826] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38826-img0003.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-38826] - Staining of human Rectum shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-38826_rabbit-polyclonal-cortactin-antibody-immunohistochemistry-paraffin-922024112033..jpg)
![Western Blot: Cortactin Antibody [NB100-855] - Analysis of Cortactin in Mouse Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NB100-855-img0001.jpg "Western Blot: Cortactin Antibody [NB100-855] - Analysis of Cortactin in Mouse Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Knockout Validated: Cortactin Antibody [NBP3-03328] - Analysis of extracts from normal (control) and CTTN knockout (KO) HeLa cells, using Cortactin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat](http://images.novusbio.com/fullsize/Cortactin-Antibody-Knockout-Validated-NBP3-03328-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Cortactin Antibody [NBP3-03328] - Analysis of extracts from normal (control) and CTTN knockout (KO) HeLa cells, using Cortactin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat")