Hu,
Mu,
Rt,
Po,
Ca,
Ch,
ChHa,
Fe,
Ma-Op,
Pm,
Am,
Bv,
Pm,
Pm-Or,
Re,
ZeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Host: Rabbit Polyclonal
Alt Catalog# IMG-6796A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27202-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27202-img0004.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0002.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0003.jpg)