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![Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP1-84129] - Adhesions are more mature and stable on PT compared to SLA. Co-localizations of tensin-1 and p-cortactin with vinculin were shown by immunocytochemical staining of HGFs cultured on PT and SLA surfaces at 24 hrs. Cells were labelled for integrin tensin-1 (red), vinculin (green), and nuclei (blue). Representative images of HGFs on PT and SLA observed under fluorescence microscopy. Grey scale images are shown for separate channels for tensin-1, and vinculin. Inserts show higher magnifications of focal contacts (white boxes). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.12451), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84129-img0013.jpg "Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP1-84129] - Adhesions are more mature and stable on PT compared to SLA. Co-localizations of tensin-1 and p-cortactin with vinculin were shown by immunocytochemical staining of HGFs cultured on PT and SLA surfaces at 24 hrs. Cells were labelled for integrin tensin-1 (red), vinculin (green), and nuclei (blue). Representative images of HGFs on PT and SLA observed under fluorescence microscopy. Grey scale images are shown for separate channels for tensin-1, and vinculin. Inserts show higher magnifications of focal contacts (white boxes). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.12451), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of rat kidneys shows positivity in mesangial cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-NBP1-84129-img0004.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84129-img0009.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of human lung shows moderate positivity.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84129-img0010.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of human smooth muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84129-img0011.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of human testis shows moderate membranous positivity.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84129-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti-Tensin 1 at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. Note the localization of Tensin1 to focal adhesion sites.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78783-img0004.jpg "Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti-Tensin 1 at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. Note the localization of Tensin1 to focal adhesion sites.")
![Western Blot: Tensin 1 Antibody [NBP2-78783] - Total protein from human U2OS and HeLa cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tensin1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Western-Blot-NBP2-78783-img0003.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP2-78783] - Analysis of a FFPE tissue section of the human kidney using 1:200 dilution of Tensin1 antibody (NBP2-78783). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly strong cytoplasmic staining in all cells in glomeruli and tubules.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-78783-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tensin1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78783-img0001.jpg)
![Western Blot: Tensin 1 Antibody [NBP1-84130] - Analysis in human cell lines U2OS and A-431 using Anti-TNS1 antibody. Corresponding TNS1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Western-Blot-NBP1-84130-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Tensin 1 Antibody [NBP1-84130] - Analysis in human cell lines U2OS and A-431 using Anti-TNS1 antibody. Corresponding TNS1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Tensin 1 Antibody [NBP1-84130] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TNS1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Western-Blot-NBP1-84130-img0008.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84130] - Staining of human kidney shows distinct membranous positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84130-img0004.jpg)
![SDS-Page: Recombinant Human Tensin 1 Protein [H00007145-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007145-P01-1.jpg "SDS-Page: Recombinant Human Tensin 1 Protein [H00007145-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Tensin 1 Antibody [NBP2-94228] - Analysis of extracts of Mouse heart, using Tensin 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Western-Blot-NBP2-94228-img0001.jpg "Western Blot: Tensin 1 Antibody [NBP2-94228] - Analysis of extracts of Mouse heart, using Tensin 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")