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![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0002.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0001.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.")
![<b>Expression of Tagged Proteins Validation. </b>Immunocytochemistry/Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Analysis of BAFF in human spleen tissue with BAFF antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76843-img0003.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Analysis of human HL60 cell lysate (H) and mouse spleen tissue lysate (M) with TNFSF13B antibody. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0001.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - HL60 cells with TNFSF13B antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76843-img0002.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NB100-56310-img0003.jpg "Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246] - Lane A: HL-60 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: MOLT-4 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDa Observed band size:32 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-182-Western-Blot-NBP2-89246-img0002.jpg "Western Blot: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246] - Lane A: HL-60 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: MOLT-4 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDa Observed band size:32 kDa (We are unsure as to the identity of these extra bands.)")
![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246] - Analysis of Human BAFF(CD257) expression on U937 cells. Cells were stained with purified anti-Human BAFF(CD257), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-182-Flow-Cytometry-NBP2-89246-img0001.jpg)
![Immunoprecipitation: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-BAFF rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-BAFF rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size: 31 kDa](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-182-Immunoprecipitation-NBP2-89246-img0003.jpg)
![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-5A8-Flow-Cytometry-NBP1-97639-img0001.jpg "Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.")
![Western Blot: BAFF/BLyS/TNFSF13B Overexpression Lysate [NBL1-17166] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF13B.](http://images.novusbio.com/fullsize/TNFSF13B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17166-img0002.jpg "Western Blot: BAFF/BLyS/TNFSF13B Overexpression Lysate [NBL1-17166] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF13B.")