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AF960

EpCAM/TROP1 Antibody [Unconjugated]

(35 Publications)

100 μg

AF960

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, MCF-7 human breast cancer cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for EpCAM/TROP-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HT‑29 human colon adenocarcinoma cell line was stained with Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

EpCAM/TROP-1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

EpCAM/TROP‑1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

EpCAM/TROP‑1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for EpCAM/TROP‑1 at approximately 52 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Jurkat human acute T cell leukemia cell line (negative) cells using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface . View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 647	FAB960R-025
Biotin	BAF960
Phycoerythrin	FAB960P-100

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF960-SP
Carrier Free	FAB960R-025
Carrier Free	FAB960P-100

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NBP2-54424

EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD) - Azide and BSA Free

100 μg

NBP2-54424

Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD) - Azide and BSA Free [NBP2-54424] - Confocal immunofluorescence analysis of MeOH-fixed HeLa cells. EpCAM/TROP1 antibody (EGP40/1555R) - (ECD) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD) - Azide and BSA Free [NBP2-54424] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD).

Flow Cytometry: EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD) - Azide and BSA Free [NBP2-54424] - Flow Cytometric Analysis of trypsinized-MCF-7 cells. EpCAM/TROP1 Antibody (EGP40/1555R) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD) - Azide and BSA Free [NBP2-54424] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Ep-CAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD) - Azide and BSA Free [NBP2-54424] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with EpCAM/TROP1 Antibody (EGP40/1555R) - (ECD).

Reactivity:

Hu, Mu(-), Rt(-)

Species Glossary

Reactivity: Hu, Mu(-), Rt(-)

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54424AF350
Alexa Fluor 405	NBP2-54424AF405
Alexa Fluor 488	NBP2-54424AF488
Alexa Fluor 532	NBP2-54424AF532
Alexa Fluor 594	NBP2-54424AF594
Alexa Fluor 647	NBP2-54424AF647
Alexa Fluor 700	NBP2-54424AF700
Alexa Fluor 750	NBP2-54424AF750
Allophycocyanin	NBP2-54424APC
Allophycocyanin/Cy7	NBP2-54424APCCY7
Biotin	NBP2-54424B
DyLight 350	NBP2-54424UV
DyLight 405	NBP2-54424V
DyLight 488	NBP2-54424G
DyLight 550	NBP2-54424R
DyLight 594	NBP2-54424DL594
DyLight 650	NBP2-54424C
DyLight 680	NBP2-54424FR
DyLight 755	NBP2-54424IR
FITC	NBP2-54424F
HRP	NBP2-54424H
Janelia Fluor 549	NBP2-54424JF549
Janelia Fluor 646	NBP2-54424JF646
PE	NBP2-54424PE
PE/Atto594	NBP2-54424PEATT594
PE/Cy5.5	NBP2-54424PECY55
PE/Cy7	NBP2-54424PECY7
PerCP	NBP2-54424PCP
mFluor Violet 450 SE	NBP2-54424MFV450
mFluor Violet 500 SE	NBP2-54424MFV500
mFluor Violet 610 SE	NBP2-54424MFV610

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