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MAB50482

CD63 Antibody (2585J) [Unconjugated]

(3 Publications)

100 μg

MAB50482

Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD63 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD63 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human PBMC were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

PBMC monocytes were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC50482U-100UG
Alexa Fluor 405	IC50482V-100UG
Alexa Fluor 488	IC50482G-100UG
Alexa Fluor 532	FAB50482X
Alexa Fluor 594	IC50482T-100UG
Alexa Fluor 647	IC50482R-100UG
Alexa Fluor 700	IC50482N-100UG
Alexa Fluor 750	IC50482S-100UG
Allophycocyanin	FAB50482A
Allophycocyanin/Cy7	FAB50482APCCY7
Biotin	FAB50482B
DyLight 350	FAB50482D
DyLight 405	FAB50482E
DyLight 488	FAB50482K
DyLight 550	FAB50482L
DyLight 594	FAB50482M
DyLight 650	FAB50482W
DyLight 680	FAB50482Y
DyLight 755	FAB50482Z
FITC	FAB50482F
HRP	FAB50482H
Janelia Fluor 549	FAB50482I
Janelia Fluor 646	FAB50482J
PE	FAB50482P
PE/Atto594	FAB50482PEATT594
PE/Cy5.5	FAB50482PECY55
PE/Cy7	FAB50482PECY7
PerCP	FAB50482C
mFluor Violet 450 SE	FAB50482MFV450
mFluor Violet 500 SE	FAB50482MFV500
mFluor Violet 610 SE	FAB50482MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB50482-SP
Carrier Free	IC50482V-100UG
Carrier Free	IC50482U-100UG
Carrier Free	IC50482T-100UG
Carrier Free	IC50482S-100UG
Carrier Free	IC50482R-100UG
Carrier Free	IC50482N-100UG
Carrier Free	IC50482G-100UG

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FAB50482MFV450

CD63 Antibody (2585J) [mFluor Violet 450 SE]

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

mFluor Violet 450 SE

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB50482U
Alexa Fluor 350	IC50482U-100UG
Alexa Fluor 405	FAB50482V
Alexa Fluor 405	IC50482V-100UG
Alexa Fluor 488	IC50482G-100UG
Alexa Fluor 488	FAB50482G
Alexa Fluor 532	FAB50482X
Alexa Fluor 594	FAB50482T
Alexa Fluor 594	IC50482T-100UG
Alexa Fluor 647	FAB50482R
Alexa Fluor 647	IC50482R-100UG
Alexa Fluor 700	IC50482N-100UG
Alexa Fluor 700	FAB50482N
Alexa Fluor 750	FAB50482S
Alexa Fluor 750	IC50482S-100UG
Allophycocyanin	FAB50482A
Biotin	FAB50482B
DyLight 350	FAB50482D
DyLight 405	FAB50482E
DyLight 488	FAB50482K
DyLight 550	FAB50482L
DyLight 594	FAB50482M
DyLight 650	FAB50482W
DyLight 680	FAB50482Y
DyLight 755	FAB50482Z
FITC	FAB50482F
HRP	FAB50482H
Janelia Fluor 549	FAB50482I
Janelia Fluor 646	FAB50482J
PE	FAB50482P
PerCP	FAB50482C
mFluor Violet 500 SE	FAB50482MFV500
mFluor Violet 610 SE	FAB50482MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB50482-100
Carrier Free	MAB50482-SP
Carrier Free	IC50482V-100UG
Carrier Free	IC50482U-100UG
Carrier Free	IC50482T-100UG
Carrier Free	IC50482S-100UG
Carrier Free	IC50482R-100UG
Carrier Free	IC50482N-100UG
Carrier Free	IC50482G-100UG

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