VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Summary
| Description |
VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.
Features:
Inhibits photobleaching of fluorescent dyes and fluorescent proteins
Ideal refractive index (1.45)
Ready-to-use - from the refrigerator to the benchtop
No warming necessary
Can be stored without sealing for long term analysis
Hardening formulation
TRITC-Phalloidin counterstain
VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.
VECTASHIELD HardSet Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. This special formulation of VECTASHIELD HardSet Mounting Medium contains TRITC-phalloidin. Phalloidin is a bicyclic heptapeptide that specifically binds at the interface between the F-actin subunits. Fluorescent derivatives of phalloidin are used to stain actin filaments.
TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence. |
Applications/Dilutions
| Dilutions |
- Cellular Imaging
- Immunocytochemistry/ Immunofluorescence
- In-situ Hybridization
|
| Application Notes |
The refractive index is 1.46.. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0028.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0015.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0016.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Tubulin (m) detected with DyLight 488 Anti-Mouse IgG and Vinculin (rp) detected with DyLight 649 Anti-Rabbit IgG. Mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0017.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Tubulin (m) detected with DyLight 488 Anti-Mouse IgG and Vinculin (rp) detected with DyLight 649 Anti-Rabbit IgG. Mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0018.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0019.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Integrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0020.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Integrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0021.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0022.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0023.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Tubulin (m) detected with DyLight 488 Anti-Mouse IgG and Vinculin (rp) detected with DyLight 649 Anti-Rabbit IgG. Mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0024.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Tubulin (m) detected with DyLight 488 Anti-Mouse IgG and Vinculin (rp) detected with DyLight 649 Anti-Rabbit IgG. Mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Integrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0025.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Integrin (m) detected with DyLight 488 Anti-Mouse IgG, mounted in a 1:1 mixture of VECTASHIELD HardSet Mounting Medium with DAPI and VECTASHIELD HardSet Mounting Medium with TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0026.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-Phalloidin-Immunocytochemistry-Immunofluorescence-H-1600-NB-img0027.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin.")
