![Western Blot: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Western blot analysis of Beta Actin (NB600-532) using v6.5 Mouse embryonic stem cells Lysate (NBP1-97433).](http://images.novusbio.com/fullsize/v6.5-Mouse-embryonic-stem-cells-Whole-Cell-Lysate-Western-Blot-NBP1-97433-img0006.jpg "Western Blot: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Western blot analysis of Beta Actin (NB600-532) using v6.5 Mouse embryonic stem cells Lysate (NBP1-97433).")
| Reactivity | MuSpecies Glossary |
| Applications | WB, IP |
| Concentration | 3.0 mg/ml |
| Dilutions |
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| Application Notes | This lysate is prepared as a positive control for immunoprecipitation or separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT loading buffer or reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS. No reducing agents or loading buffer added. |
| Preservative | No Preservative |
| Concentration | 3.0 mg/ml |
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![SDS-Page: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Ponceau Stain: Staining of v6.5 Mouse embryonic stem cells Lysate using NBP1-97433.](http://images.novusbio.com/fullsize/v6.5-Mouse-embryonic-stem-cells-Whole-Cell-Lysate-SDS-Page-NBP1-97433-img0004.jpg "SDS-Page: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Ponceau Stain: Staining of v6.5 Mouse embryonic stem cells Lysate using NBP1-97433.")