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V5 Epitope Tag Antibody [Janelia Fluor® 669]

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Product Details

Summary
Reactivity ETSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Janelia Fluor 669

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V5 Epitope Tag Antibody [Janelia Fluor® 669] Summary

Immunogen
Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for V5 Epitope Tag Antibody [Janelia Fluor® 669]

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for V5 Epitope Tag Antibody (NB600-380JF669) (0)

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Secondary Antibodies

 

Isotype Controls

Additional V5 Epitope Tag Products

Array NB600-380JF669

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

mFluor Violet Conjugated Antibodies

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