Ultra Rainbow Calibration ERF Particle Set (6 peaks) Summary
| Description |
Ultra Rainbow Quantitative Particle Kit
Contains 6 intensities of Ultra Rainbow Fluorescent Beads with NIST assigned ERF (Equivalent Number of Reference Fluorophores) values based on a published procedure using NIST SRM 1934 and a calibrated laserbased CCD fluorimeter.
Provides microsphere reference standards for fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC) and Pacific Blue (PB) fluorescent channels using four different NIST reference fluorophores; fluorescein, Nile Red, APC and Coumarin 30.
Used during flow cytometry standardardization and calibration measurements for the further advancement of biological and clinical applications.
Particle size: 3.5 -3.9 micron
Size: 6 x 2 mL |
Applications/Dilutions
| Dilutions |
- Flow Cytometry
- Fluorescence Imaging
|
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. Do not freeze. |
Background
Rainbow Calibration Particles are used to ascertain the relative voltage range for each flow cytometer detector. This mixture of fluorescent particles serves as a reliable and reproducible means of evaluating the sensitivity and resolution of flow cytometers.
The relative number of fluorophores can been determined for every emission peak of the rainbow calibration particles in FL1 (FITC, MEFL), FL2 (RPE, MEPE), FL3 (RPE-Cy5, MEPCY) and FL4 (APC, MEAP) channels in order to plot the calibration graph. The calibration graph is used to check the linearity (dynamic range) of the photomultiplier tube (PMT) in each channel. Additionally, the relative number of fluorophores can be cross calibrated with cells or particles stained with a known number of spectral matching fluorophores such as FITC, PE, RPE-Cy5 to estimate the number of fluorophores on stained cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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