![SDS-Page: Total Protein Extraction Kit [NBP2-37853] - 50 ug total proteins on 4-20% gradient SDS-PAGE gel. Total proteins were isolated from 8 different human tissues. Lane 1, Liver; lane 2, Kidney; lane 3, Brain; lane 4, Skeletal Muscle; lane 5 Spleen; lane 6, Lung; lane 7, Placenta; and lane 8, Stomach](http://images.novusbio.com/fullsize/Total-Protein-Extraction-Kit-SDS-Page-NBP2-37853-img0004.jpg "SDS-Page: Total Protein Extraction Kit [NBP2-37853] - 50 ug total proteins on 4-20% gradient SDS-PAGE gel. Total proteins were isolated from 8 different human tissues. Lane 1, Liver; lane 2, Kidney; lane 3, Brain; lane 4, Skeletal Muscle; lane 5 Spleen; lane 6, Lung; lane 7, Placenta; and lane 8, Stomach")
| Description | Novus biologicals Total Protein Extraction Kit is an excellent tool for purification and preparation of proteins from tissues or cells. Total protein isolated with this kit has native conformation and can be used for downstream applications such as: SDS-PAGE, Western Blotting, protein assays and other procedures. This kit is easy to use: no scraping, no freeze-thaw cycles, and no sonication. Fast and easy protocol - generate total protein samples in 45 minutes. Isolate protein from large samples of tissue or cultured cells. Reagent is provided for up to 5.2 grams of tissue or 130 million cultured cells. Rapid isolation without freeze-thaw and sonication. Proteins isolated by this kit are intact and could be over 200 kd in mass. |
| Kit Type | Extraction Kit |
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| Application Notes | The Total Protein Extraction Kit is composed of two buffers: TM and 50X PI. Before isolation of total proteins from tissues or cells, simply dilute the 50X PI buffer with TM buffer according to the instruction in the manual. The kit can be used for isolation of total proteins from 5 gram of tissue or 250 million cultured cells. Recommended minimal 0.1 g tissue or 2.5 million cells per reaction, but for some high protein yield tissue or cells, minimal amount of specimen can be even smaller with proportionally less reagent. |
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| Storage | Storage is content dependent. |
| Buffer | 50 x PI: A cocktail of protease inhibitors; 260 ul TM Buffer: HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, SodiumOrthoVanadate; 13 ml |
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