Thrombomodulin/BDCA-3 Antibody (4D8.2C6) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC
Clone
4D8.2C6
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 700

Order Details

Thrombomodulin/BDCA-3 Antibody (4D8.2C6) [Alexa Fluor® 700] Summary

Immunogen
Two synthetic peptides made to internal portions of the mouse Thrombomodulin protein (between residues 25-150 and 400-550) [UniProt P07204]
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
THBD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Thrombomodulin/BDCA-3 Antibody (4D8.2C6) [Alexa Fluor® 700]

  • BDCA3
  • BDCA-3
  • CD141 antigen
  • CD141
  • Fetomodulin
  • THBD
  • THRM
  • Thrombomodulin
  • TM

Background

CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Thrombomodulin/BDCA-3.

Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

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Bioinformatics

Gene Symbol THBD