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Tangential Flow Filtration (TFF)-EV Concentrator

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Product Details

Summary
Suitable Sample Type
Diluted matrices (urine and cell culture supernatant)

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Tangential Flow Filtration (TFF)-EV Concentrator Summary

Description

Tangential flow filter-Simple (TFF-Simple) are a filter cartridge of hollow polysulfone fibers used for rapid concentration of dilute samples, such as urine and cell culture media prior to EV isolation. The tangential flow filtration systems allows for efficient concentration of EVs without impacting vesicle shape or function. No time-consuming ultracentrifugation step, limited filter clogging compared to traditional diafiltration, concentrated samples can be directly loaded onto SEC columns or used in other EV isolation techniques, ability to dialyze EV preparations, filters can be reused multiple times.

Sample Volume
5-15 mL

Applications/Dilutions

Publications
Read Publication using NBP3-11762.

Packaging, Storage & Formulations

Storage
Store at room temperature.

Background

Tangetial flow filtration (TFF) is a technique primarily used for concentrating large sample volumes containing extracellular vesicles (EVs). The process involves passing the EV-containing liquid horizontally (tangentially) over the pores of the membrane allowing particles that are smaller than the membrane pores to filter through, while larger particles are retained in the chamber.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Tangential Flow Filtration (TFF)-EV Concentrator (NBP3-11762)(1)

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