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Human Skin Whole Tissue Lysate (Adult Whole Diabetes)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
5.0 mg/ml

Order Details

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Human Skin Whole Tissue Lysate (Adult Whole Diabetes) Summary

Description
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Specificity
Total Protein - Human Diabetic Diseased Tissue: Skin

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Skin
Protein State
Native
Tissue Condition
Diabetes
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Skin Lysate (NB820-59295). (Showing 1 - 1 of 1 FAQ).

  1. For adult normal frozen skin tissue slides can I choose the donor age/sex? 
    • Unfortunately, that is not an option for our commercially available tissue slides.

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Calculators

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