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Human Skin Whole Tissue Lysate (Adult Whole Normal)

Images

 
SDS-Page: Human Skin Whole Tissue Lysate (Adult Whole Normal) [NB820-59254] - man Skin Whole Tissue Lysate (Whole Normal) [NB820-59254] - Protein lysate is verified on SDS-PAGE analysis as a consistent pattern.
Western Blot: Human Skin Whole Tissue Lysate (Adult Whole Normal) [NB820-59254] - Human skin Whole Tissue Lysate (Adult Whole Normal) 30ug/lane was loaded to SDS-PAGE gel and transferred to a membrane. Image from a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
5.0 mg/ml

Order Details

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Human Skin Whole Tissue Lysate (Adult Whole Normal) Summary

Description
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Specificity
Total Protein - Human Adult Normal Tissue: Skin

Applications/Dilutions

Dilutions
  • Western Blot 10 ug -20 ug
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Reviewed Applications
Read 1 Review rated 5
using
NB820-59254 in the following application:

Publications
Read Publications using
NB820-59254 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Skin
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Skin Lysate (NB820-59254)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(1)
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Human
(1)
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Review for Skin Lysate (NB820-59254) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NB820-59254:
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Filter by Species
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Images Ratings Applications Species Date Details
  5
reviewed by:
Ming Lu
05/15/2024
View

Summary

LotC808027

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Skin Lysate (NB820-59254). (Showing 1 - 1 of 1 FAQs).

  1. For adult normal frozen skin tissue slides can I choose the donor age/sex? 
    • Unfortunately, that is not an option for our commercially available tissue slides.

Additional Skin Products

Blogs on Skin

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Contact Information

Product PDFs

Calculators

Concentration Calculator

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ming Lu
05/15/2024
Application:
Species: