Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Diabetes) Summary
| Description |
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity |
Total Protein - Human Diabetic Diseased Tissue: Skeletal Muscle |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration |
5.0 mg/ml |
Lysate Details for Array
| Type |
Tissue |
| Life Stage |
Adult |
| Tissue |
Skeletal Muscle |
| Protein State |
Native |
| Tissue Condition |
Diabetes |
| Subcellular Fraction |
Whole |
Background
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
Publications for Skeletal Muscle Lysate (NB820-59294) (0)
There are no publications for Skeletal Muscle Lysate (NB820-59294).
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Skeletal Muscle Lysate (NB820-59294). (Showing 1 - 2 of 2 FAQ).
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I am interested in your skeletal muscle total protein, including NB820-59294, NB820-59253 and NBL1-26511. I would like to know if you have the skeletal muscle tissue from different individuals? Are all of they from the same people? How detailed information about the donor could you offer us? Could you give us the body weight, height, blood glucose of the donor? Do you know the diabetic donor is type 1 or type 2 diabetes? Do you have any scientific papers using this product?
- NB820-59294 comes from a 67 year old male donor. NB820-59253 comes from a 24 year old male donor. NBL1-26511 comes from a 79 year old male donor. This patient had type II diabetes. That is the only other information I have on the patient at this time. I also have no publications using this product at the moment.
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why the composition of the buffer for the diabetic patient is different?
- The normal tissue sample (NBP2-25037) was originally an Imgenex product. After our aquisition, we brought this product into our product line. We kept their formulation. The diabetic patient (NB820-59294) was one of our original tissue lysates. As we were sourcing the donors from a separate source than the Imgenex product, the formulations were different.
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