Applications/Dilutions
| Dilutions |
- Chromatin Immunoprecipitation (ChIP) 0.5 uM
|
| Application Notes |
This SAT2 primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 80 base pairs. |
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
10 uM in TE buffer. |
| Preservative |
No Preservative |
Background
Primers are designed to amplify DNA from human Sat2 repeats in chromosome 1. Sat2 repeats are found in centromeric regions of chromosomes and associated with heterochromatin.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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Blogs on Satellite2 repeat primer
![Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/Satellite2-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71655-img0009.jpg "Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.")
![Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - 2 ug of NB21-1073 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Satellite2-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71655-img0006.jpg "Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - 2 ug of NB21-1073 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.](http://images.novusbio.com/fullsize/Satellite2-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71655-img0007.jpg "Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.")
