SARS-CoV-2 Spike RBD Antibody (1038345) [Alexa Fluor® 647] Summary
| Immunogen |
Chinese Hamster Ovary cell line CHO-derived MERS-CoV Spike RBD
Glu367-Tyr606
Accession # YP_007188579.1 |
| Specificity |
Detects MERS-CoV Spike RBD in direct ELISAs. |
| Source |
N/A |
| Isotype |
IgG2a |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Packaging, Storage & Formulations
| Storage |
Protect from light. Do not freeze.- 12 months from date of receipt, 2 to 8 °C as supplied.
|
| Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
| Reconstitution Instructions |
Reconstitution default not known |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for SARS-CoV-2 Spike RBD Antibody (1038345) [Alexa Fluor® 647]
Background
MERS-CoV (also known as HCoV-EMC), which causes the Middle East
Respiratory Syndrome (MERS), was first reported in Saudi Arabia in 2012 as a
novel coronavirus (1). Coronaviruses are a family of viruses that are commonly
comprised of a large plus-strand RNA genome and four structural proteins: Spike
protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid
protein (N). There are two well-known human coronavirus families that infect
humans: Alpha coronaviruses which includes HCoV-229E and HCoV-NL63; beta
coronaviruses that includes MERS-CoV, HCov-OC43, Severe Acute Respiratory
Syndrome (SARS-CoV), and global pandemic Covid-19 (SARS-CoV2) (2). The MERS-CoV Spike Protein (S
Protein) is a glycoprotein that mediates membrane fusion and viral entry, and
it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment
of the protein to the host receptor while the S2 subunit is involved with cell
fusion (3). Located within the
S1 subunit is the receptor binding domain (RBD). The RBD is responsible for the
binding of MERS-CoV to dipeptidyl peptidase IV (DPP4, also known as human CD26)
(4). The RBD of MERS-CoV shares 24% and 21% amino acid sequence (aa) identity with SARS-CoV RBD and
SARS-Cov2 RBD, respectively. The low aa sequence identity is consistent with
the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). The
S1 subunit, especially the RBD region, of MERS-CoV was commonly targeted for
vaccinations or antiviral therapies (5-7).
- Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
- Ogimi, C. et al. (2020) J Pediatric Infect Dis Soc doi: 10.1093/jpids/piaa037.
- Li, Y. et al. (2019) Engineering. 5:940.
- Raj, V.S. et al. (2013) Nature 495:251.
- Corti, D. et al. (2016) J. Infect. Public Health 9:231.
- Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
- Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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