Plant Rubisco ELISA Kit (Colorimetric) Summary
| Specificity |
This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed. |
| Standard Curve Range |
3.12 ug/mL - 800 ug/mL |
| Sensitivity |
3.12 ug/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
%CV < 20 |
| Intra-Assay |
%CV < 15 |
| Sample Volume |
50 - 100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
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Components
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- Adhesive Strip (For 96 wells)
- Antibody (100 x concentrate)
- Antibody Diluent
- HRP-conjugate Diluent
- HRP-conjugate(100 x concentrate)
- Instruction manual
- Sample Diluent
- Sample Extraction Buffer (25 x concentrate)
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Plant Rubisco ELISA Kit (Colorimetric)
Background
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO. The competitive inhibition reaction is launched between with pre-coated RuBisCO and RuBisCO in samples with the antibody. Then add a Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit IgG antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on Rubisco
![ELISA: Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Plant-Rubisco-ELISA-Kit-Colorimetric-ELISA-NBP2-60142-img0001.jpg "ELISA: Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-60142_plant-rubisco-elisa-kit-colorimetric-312024831592.png "Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] - Standard Curve Reference")
