GC376 achieved 50% inhibition of 50 nM SARS-CoV Virus 3CL Protease (Catalog # E-718) at a concentration of 101-120 nM.
1 μg and 2 μg of Recombinant SARS-CoV Virus 3CL Protease (Catalog # E-718) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 33 kDa.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using E-718 in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant SARS-CoV 3CL Protease, CF
3CL Protease
3CL Proteinase
3C-like main protease
3CL-Mpro
COVID-19
M Proteinase
Main Protease
Mpro
Background
The 3CL protease (aka 3CLpro, Mpro or "Main Protease) from the human SARS-CoV coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide. 3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid. Along with the CoV Papain-Like Protease (catalog number E-610), 3CLpro presents an attractive target for therapeutic intervention of coronavirus infections. Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity.
Chen, Y.W., et al. (2020) F1000Research 9:129.
Pillaiyar, T., et al. (2016) J. Med. Chem. 59:6595.
Publications for SARS-CoV-2 3CL Protease (E-718)(2)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 1 application: Bioassay.
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