Recombinant Mouse TLR12 Fc Chimera Protein, CF Summary
| Details of Functionality |
Measured by its binding ability in a functional ELISA. When recombinant T. gondii Profilin is immobilized at 5 µg/mL (100 µL/well), the concentration of Recombinant Mouse TLR12 Fc Chimera that produces 50% of the optimal binding response is approximately 5-25 ng/mL |
| Source |
Chinese Hamster Ovary cell line, CHO-derived mouse TLR12 protein
Mouse TLR12
(Thr22-Lys709)
Accession # NP_991392 |
IEGRMDP |
Mouse IgG2A
(Glu98-Lys330) |
| N-terminus |
|
C-terminus |
|
|
| Accession # |
|
| N-terminal Sequence |
Thr22 |
| Structure / Form |
Disulfide-linked homodimer |
| Protein/Peptide Type |
Recombinant Proteins |
| Gene |
Tlr12 |
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
102 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE |
105-125 kDa, reducing conditions |
Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Reconstitution Instructions |
Reconstitute at 100 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse TLR12 Fc Chimera Protein, CF
Background
Toll-like receptors (TLRs) are type I transmembrane proteins that activate the immune system in response to molecular patterns derived from microbial pathogens. Some TLRs are expressed on the cell surface (TLR1, 2, 4, 5, 6, 10), while others are found in endosomal structures (TLR3, 7, 8, 9, 11, 12, 13). TLRs contain a large number of leucine-rich repeats (LRRs) in their ectodomains and a cytoplasmic tail with one signal transducing Toll/IL-1 receptor (TIR) domain (1, 2). Mature mouse TLR12 is an approximately 100 kDa glycoprotein that consists of a 688 amino acid (aa) ectodomain with 17 LRRs, a 21 aa transmembrane segment, and a 176 aa cytoplasmic region (3). Within the ectodomain, mouse TLR12 shares 80% aa sequence identity with rat TLR12. TLR12 is expressed in dendritic cells, macrophages, and neurons, and in the uterus, liver, kidney, and bladder (3-6). It is up-regulated in the adipose tissue of obesity-prone ob/ob mice fed a high fat diet (7). The intracellular trafficking and sorting of several TLRs (including TLR12) is dependent on the endoplasmic reticulum resident protein UNC93B1 (8, 9). TLR12 can associate into homodimers as well as heterodimers with TLR11 (4). TLR11 can additionally heterodimerize with TLR3 and TLR7 (4, 8). TLR12 is required for the inflammatory response against uropathogenic bacteria and
Toxoplasma gondii (3, 4). It directly binds Profilin from
T. gondii, and it cooperates with TLR11 in protecting against
T. gondii infection
in vivo (4).
- Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
- Song, D.H. and J.O. Lee (2012) Immunol. Rev. 250:216.
- Zhang, D. et al. (2004) Science 303:1522.
- Koblansky, A.A. et al. (2013) Immunity 38:119.
- Mishra, B.B. et al. (2008) J. Neuroinflam. 5:53.
- Hickey, D.K. et al. (2013) Innate Immun. 19:121.
- Kim, S.J. et al. (2012) J. Nutr. Biochem. 23:113.
- Andrade, W.A. et al. (2013) Cell Host Microbe 13:42.
- Lee, B.L. et al. (2013) eLife 2:e00291.
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