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Recombinant Mouse IL-27 p28/IL-30 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
7430-ML-010
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Recombinant Mouse IL-27 p28/IL-30 Protein, CF Summary

Details of Functionality
Measured by its ability to induce CXCL10/IP‑10 secretion by THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is < 35 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-27 p28/IL-30 protein
Phe29-Ser234, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Phe29
Protein/Peptide Type
Recombinant Proteins
Gene
Il27
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
25-35 kDa, reducing conditions
Publications
Read Publications using
7430-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and CHAPS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-27 p28/IL-30 Protein, CF

  • IL27 p28
  • IL-27 p28
  • IL27;IL-27;IL27A;IL-27A;IL27p28;IL-27p28;IL30;p28
  • IL-30

Background

Interleukin‑27 p28, also known as IL‑30, is a secreted 28 kDa protein that is considered a member of the IL‑6/IL‑12 interfamily cytokine family. p28/IL‑30 is one of several alpha ‑chain proteins that can associate with various beta ‑chain proteins to form heterodimeric cytokines in these families. The alpha -chains (e.g. IL‑6, IL‑11, Cardiotrophin‑1, CLC/CNTF, LIF, Oncostatin M, IL‑23 p19, IL‑27 p28/IL‑30, IL‑12/IL‑35 p35) have a four‑helix bundle structure, while the beta ‑chains (e.g. EBI‑3, CLF‑1, IL‑12/IL‑23 p40) resemble class 1 cytokine receptors. These cytokines utilize heteromeric cell surface receptors which contain shared as well as ligand‑specific subunits. Divergent biological responses are obtained from the combinatorial association of cytokine subunits and their interaction with various combinations of receptor subunits. Complexity in this system is increased by the generation of soluble receptors and by the competition between proteins for shared subunit pairing (1‑3). p28/IL‑30 is expressed by macrophages and dendritic cells, and is up‑regulated in these cells by inflammatory stimuli (4‑9). It was first described as a partner with EBI‑3 in the heterodimeric cytokine IL‑27 (4). IL‑27 signals through a receptor complex composed of IL‑27 R alpha /WSX-1/TCCR and gp130 (4, 10, 11). This interaction enhances the proinflammatory activation of naïve CD4+ T cells, NK cells, mast cells, and monocytes and the cytotoxic activity of CD8+ T cells (4, 10, 12). IL‑27 also exhibits anti-inflammatory activity, including the induction of IL‑10 production by naïve and memory T cells, the activation of regulatory T cells (Treg), and the suppression of Th17 cytokine secretion (13, 14). Alternatively, p28/IL‑30 associates with CLF‑1 to create a cytokine that triggers responses through IL‑27 R alpha , IL‑6 R alpha , and gp130 (6). Like IL‑27, p28‑CLF-1 heterodimers co‑stimulate IFN‑ gamma production by NK cells, and induce IL‑10 secretion by CD4+ T cells (6). In contrast to IL‑27, however, p28‑CLF-1 is reported to promote the differentiation of Th17 cells (6). A third mode of p28 action enables it to stimulate cells that express both IL‑6 R alpha and gp130, but lack IL‑27 R alpha (11). Similar to the IL‑6 system, the presence of IL‑6 R alpha on the cell surface is not even required if p28/IL‑30 associates with a soluble form of IL‑6 R alpha . This combination can trigger trans signaling through gp130, a mechanism that has been demonstrated for complexes of IL‑6 with soluble IL‑6 R alpha (11). Over‑expression of p28/IL‑30 in vivo interferes with humoral antibody responses and protects from IL‑12 induced liver inflammation (7, 15). Mature mouse p28/IL-30 shares 70% and 89% amino acid sequence identity with human and rat p28, respectively
  1. Hunter, C.A. and R. Kastelein (2012) Immunity 37:960.
  2. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  3. Vignali, D.A.A. and V.K. Kuchroo (2012) Nat. Immunol. 13:722.
  4. Pflanz, S. et al. (2002) Immunity 16:779.
  5. Liu, J. et al. (2007) J. Exp. Med. 204:141.
  6. Crabé, S. et al. (2009) J. Immunol. 183:7692.
  7. Stumhofer, J.S. et al. (2010) Nat. Immunol. 11:1119.
  8. Bosmann, M. et al. (2012) J. Immunol. 188:5086.
  9. Dibra, D. et al. (2012) J. Immunol. 188:3709.
  10. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
  11. Garbers, C. et al. (2013) J. Biol. Chem. 288:4346.
  12. Schneider, R. et al. (2011) Eur. J. Immunol. 41:47.
  13. Diveu, C. et al. (2009) J. Immunol. 182:5748.
  14. Hall, A.O, et al. (2012) Immunity 37:511.
  15. Dibra, D. et al. (2012) Hepatology 55:1204.

Publications for IL-27 p28/IL-30 (7430-ML/CF)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Il27
Uniprot