Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Ephrin‑B2, also known as Htk‑L, ELF‑2, LERK‑5, and NLERK‑1, is a 40 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin‑B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin‑B2 shares 97% and 98% aa sequence identity with human and rat Ephrin‑B2, respectively. Ephrin‑B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6‑8). Ephrin‑B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8‑11). Ephrin‑B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14‑16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin‑B2 is also important for the separation of the urinary and intestinal tracts during development (18).

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Avian - Quail, Chicken, Primate - Chlorocebus aethiops (African Green Monkey), Virus, Virus - Ghana, Virus - Henipavirus.

We have publications tested in 14 applications: Binding Assay, Bioassay, Cell Culture, Co-Immunoprecipitation, Coprecipitation Assay, ELISA Capture, Enzyme Assay, Flow Cytometry, ICC, IHC, IHC-Fr, Immunoprecipitation, In Vivo, Western Blot.

Showing Publications 1 - 10 of 46. Show All 46 Publications. Collapse Publications.

Publications using 496-EB	Applications	Species
Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A; Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693478] (Bioassay, Human, Chicken)	Bioassay	Human, Chicken
M Yang, W Zhu, T Truong, B Pickering, S Babiuk, D Kobasa, L Banadyga Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays Viruses, 2022-07-28;14(8):. 2022-07-28 [PMID: 36016279] (ELISA Capture, Mouse)	ELISA Capture	Mouse
S Bhatia, D Nguyen, LB Darragh, B Van Court, J Sharma, MW Knitz, M Piper, S Bukkapatna, J Gadwa, TE Bickett, S Bhuvane, S Corbo, B Wu, Y Lee, M Fujita, M Joshi, LE Heasley, RL Ferris, O Rodriguez, C Albanese, M Kapoor, EB Pasquale, SD Karam EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment Nature Communications, 2022-06-20;13(1):3535. 2022-06-20 [PMID: 35725568] (Bioassay, Mouse)	Bioassay	Mouse
V Ortega, JLR Zamora, IA Monreal, DT Hoffman, S Ezzatpour, GP Johnston, EM Contreras, FJ Vilchez-De, HC Aguilar Novel Roles of the Nipah Virus Attachment Glycoprotein and Its Mobility in Early and Late Membrane Fusion Steps MBio, 2022-05-04;0(0):e0322221. 2022-05-04 [PMID: 35506666] (Bioassay, Mouse)	Bioassay	Mouse
D Chen, ED Hughes, TL Saunders, J Wu, MN Hernández, T Makinen, PD King Angiogenesis depends upon EPHB4-mediated export of collagen IV from vascular endothelial cells JCI Insight, 2022-02-22;0(0):. 2022-02-22 [PMID: 35015735] (Bioassay, Mouse)	Bioassay	Mouse
C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 2020-09-08;32(10):108105. 2020-09-08 [PMID: 32905777] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo Oxid Med Cell Longev, 2020-06-05;2020(0):1404915. 2020-06-05 [PMID: 32587656] (Bioassay, Mouse)	Bioassay	Mouse
ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus)	Coprecipitation Assay	Virus - Henipavirus
K Voigt, M Hoffmann, JF Drexler, MA Müller, C Drosten, G Herrler, N Krüger Fusogenicity of the Ghana Virus (Henipavirus: Ghanaian bat henipavirus) Fusion Protein is Controlled by the Cytoplasmic Domain of the Attachment Glycoprotein Viruses, 2019-08-29;11(9):. 2019-08-29 [PMID: 31470664] (Immunoprecipitation, Virus - Ghana)	Immunoprecipitation	Virus - Ghana
D Herrero, S Cañón, G Albericio, RM Carmona, S Aguilar, S Mañes, A Bernad Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart Redox Biol, 2019-03-04;22(0):101156. 2019-03-04 [PMID: 30851670] (Bioassay, Flow Cytometry, Mouse)	Bioassay, Flow Cytometry	Mouse
JM Baek, YH Cheon, SC Kwak, HY Jun, KH Yoon, MS Lee, JY Kim Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling Exp. Mol. Med., 2018-04-27;50(4):50. 2018-04-27 [PMID: 29700355] (Bioassay, Mouse)	Bioassay	Mouse
E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 2018-04-11;0(0):. 2018-04-11 [PMID: 29643120] (Cell Culture, In Vivo, Human, Mouse)	Cell Culture, In Vivo	Human, Mouse
B Jakob, G Kochlamaza, M Jäpel, A Gauhar, HH Bock, T Maritzen, V Haucke Intersectin 1 is a component of the Reelin pathway to regulate neuronal migration and synaptic plasticity in the hippocampus Proc. Natl. Acad. Sci. U.S.A., 2017-05-08;114(21):5533-5538. 2017-05-08 [PMID: 28484035] (Bioassay, Mouse)	Bioassay	Mouse
The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model Mol Psychiatry, 2016-09-20;0(0):. 2016-09-20 [PMID: 27646263] (Bioassay, Rat)	Bioassay	Rat
M Wang, MJ Collins, TR Foster, H Bai, T Hashimoto, JM Santana, C Shu, A Dardik Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase J. Vasc. Surg., 2016-01-24;65(1):179-189. 2016-01-24 [PMID: 26817610] (Bioassay, Mouse)	Bioassay	Mouse
Miyamoto T, Kim D, Knox J, Johnson E, Mucke L Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-beta-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity. J Biol Chem, 2015-11-20;291(4):1719-34. 2015-11-20 [PMID: 26589795] (Bioassay, Mouse)	Bioassay	Mouse
Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 2015-09-25;10(9):e0138835. 2015-09-25 [PMID: 26407069] (Bioassay, Mouse)	Bioassay	Mouse
Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Enzyme Assay, Mouse)	Enzyme Assay	Mouse
Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model. J Neurosci, 2015-04-01;35(13):5409-21. 2015-04-01 [PMID: 25834064] (Co-Immunoprecipitation, Rat)	Co-Immunoprecipitation	Rat
Lisle J, Mertens-Walker I, Stephens C, Stansfield S, Clements J, Herington A, Stephenson S Murine, but not human, ephrin-B2 can be efficiently cleaved by the serine protease kallikrein-4: implications for xenograft models of human prostate cancer. Exp Cell Res, 2015-02-24;333(1):136-46. 2015-02-24 [PMID: 25724897] (Bioassay, Mouse)	Bioassay	Mouse
Liu Q, Bradel-Tretheway B, Monreal A, Saludes J, Lu X, Nicola A, Aguilar H Nipah virus attachment glycoprotein stalk C-terminal region links receptor binding to fusion triggering. J Virol, 2014-11-26;89(3):1838-50. 2014-11-26 [PMID: 25428863] (Bioassay, Virus)	Bioassay	Virus
Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R The composition of EphB2 clusters determines the strength in the cellular repulsion response. J Cell Biol, 2014-01-27;204(3):409-22. 2014-01-27 [PMID: 24469634] (Bioassay, Human, Primate - Chlorocebus aethiops (African Green Monkey))	Bioassay	Human, Primate - Chlorocebus aethiops (African Green Monkey)
Conway A, Schaffer D Biomaterial microenvironments to support the generation of new neurons in the adult brain. Stem Cells, 2014-05-01;32(5):1220-9. 2014-05-01 [PMID: 24449485] (Bioassay)	Bioassay
van Gils , Janine M, Ramkhelawon , Bhama, Fernandes , Luciana, Stewart , Merran C, Guo , Liang, Seibert , Tara, Menezes , Gustavo, Cara , Denise C, Chow , Camille, Kinane , T Bernar, Fisher , Edward A, Balcells , Mercedes, Alvarez-Leite , Jacqueli, Lacy-Hulbert , Adam, Moore , Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9. 2013-02-21 [PMID: 23430612] (Bioassay, Human)	Bioassay	Human
Soskis , Michael, Ho , Hsin-Yi, Bloodgood , Brenda L, Robichaux , Michael, Malik , Athar N, Ataman , Bulent, Rubin , Alex A, Zieg , Janine, Zhang , Chao, Shokat , Kevan M, Sharma , Nikhil, Cowan , Christop, Greenberg , Michael A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development. Nat Neurosci, 2012-11-11;15(12):1645-54. 2012-11-11 [PMID: 23143520] (Bioassay, Mouse)	Bioassay	Mouse
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012-05-23;42(6):1562-72. 2012-05-23 [PMID: 22622783] (Bioassay, Mouse)	Bioassay	Mouse
Maddigan A, Truitt L, Arsenault R EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes. J. Immunol., 2011-10-28;187(11):5983-94. 2011-10-28 [PMID: 22039307] (Bioassay, Human)	Bioassay	Human
Naska S, Lin DC, Miller FD p75NTR is an obligate signaling receptor required for cues that cause sympathetic neuron growth cone collapse. Mol. Cell. Neurosci., 2010-06-17;45(2):108-20. 2010-06-17 [PMID: 20584617] (Bioassay, Mouse)	Bioassay	Mouse
Genander M, Holmberg J, Frisen J Ephrins negatively regulate cell proliferation in the epidermis and hair follicle. Stem Cells, 2010-07-01;28(7):1196-205. 2010-07-01 [PMID: 20506314] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis. Nature, 2010-05-05;465(7297):487-91. 2010-05-05 [PMID: 20445540] (Bioassay, Mouse)	Bioassay	Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010-03-24;30(12):4246-55. 2010-03-24 [PMID: 20335460] (Bioassay, Rat)	Bioassay	Rat
He S, Kumar SR, Zhou P, Krasnoperov V, Ryan SJ, Gill PS, Hinton DR Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro. Invest. Ophthalmol. Vis. Sci., 2009-08-20;51(1):543-52. 2009-08-20 [PMID: 19696168] (Bioassay, Human)	Bioassay	Human
Pennisi A, Ling W, Li X, Khan S, Shaughnessy JD, Barlogie B, Yaccoby S The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth. Blood, 2009-07-13;114(9):1803-12. 2009-07-13 [PMID: 19597185] (In Vivo, Human)	In Vivo	Human
Slack S, Battaglia A, Cibert-Goton V, Gavazzi I EphrinB2 induces tyrosine phosphorylation of NR2B via Src-family kinases during inflammatory hyperalgesia. Neuroscience, 2008-07-18;156(1):175-83. 2008-07-18 [PMID: 18694808] (In Vivo, Rat)	In Vivo	Rat
Mansson-Broberg A, Siddiqui AJ, Genander M, Grinnemo KH, Hao X, Andersson AB, Wardell E, Sylven C, Corbascio M Modulation of ephrinB2 leads to increased angiogenesis in ischemic myocardium and endothelial cell proliferation. Biochem. Biophys. Res. Commun., 2008-06-20;373(3):355-9. 2008-06-20 [PMID: 18571496] (In Vivo, Mouse)	In Vivo	Mouse
Masuda J, Usui R, Maru Y Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis. J. Biol. Chem., 2008-02-28;283(19):13148-55. 2008-02-28 [PMID: 18308734] (Bioassay, Human)	Bioassay	Human
Nestor MW, Mok LP, Tulapurkar ME, Thompson SM Plasticity of neuron-glial interactions mediated by astrocytic EphARs. J. Neurosci., 2007-11-21;27(47):12817-28. 2007-11-21 [PMID: 18032653] (Bioassay, Mouse)	Bioassay	Mouse
Negrete OA, Chu D, Aguilar HC, Lee B Single amino acid changes in the Nipah and Hendra virus attachment glycoproteins distinguish ephrinB2 from ephrinB3 usage. J. Virol., 2007-07-25;81(19):10804-14. 2007-07-25 [PMID: 17652392] (Bioassay, Human)	Bioassay	Human
Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth. Biochemistry, 2007-01-24;46(7):1799-810. 2007-01-24 [PMID: 17249693] (Binding Assay, Human)	Binding Assay	Human
Yang NY, Pasquale EB, Owen LB, Ethell IM The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization. J. Biol. Chem., 2006-08-31;281(43):32574-86. 2006-08-31 [PMID: 16950769] (Bioassay, Immunoprecipitation, Mouse)	Bioassay, Immunoprecipitation	Mouse
Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J EphB receptors coordinate migration and proliferation in the intestinal stem cell niche. Cell, 2006-06-16;125(6):1151-63. 2006-06-16 [PMID: 16777604] (In Vivo, Mouse)	In Vivo	Mouse
Masood R, Kumar SR, Sinha UK, Crowe DL, Krasnoperov V, Reddy RK, Zozulya S, Singh J, Xia G, Broek D, Schonthal AH, Gill PS EphB4 provides survival advantage to squamous cell carcinoma of the head and neck. Int. J. Cancer, 2006-09-15;119(6):1236-48. 2006-09-15 [PMID: 16615113] (Bioassay, Mouse)	Bioassay	Mouse
Ricard J, Salinas J, Garcia L, Liebl DJ EphrinB3 regulates cell proliferation and survival in adult neurogenesis. Mol. Cell. Neurosci., 2006-02-17;31(4):713-22. 2006-02-17 [PMID: 16483793] (In Vivo, Mouse)	In Vivo	Mouse
le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, Breant C, Fleury V, Eichmann A Flow regulates arterial-venous differentiation in the chick embryo yolk sac. Development, 2003-12-17;131(2):361-75. 2003-12-17 [PMID: 14681188] (IHC, In Vivo, Avian - Quail, Chicken)	IHC, In Vivo	Avian - Quail, Chicken
Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J. Cell. Sci., 2003-05-06;116(0):2461-70. 2003-05-06 [PMID: 12734395] (Bioassay, ICC, IHC, Human)	Bioassay, ICC, IHC	Human
Sharfe N, Freywald A, Toro A, Dadi H, Roifman C Ephrin stimulation modulates T cell chemotaxis. Eur. J. Immunol., 2002-12-01;32(12):3745-55. 2002-12-01 [PMID: 12516569] (Bioassay, Human)	Bioassay	Human
Show All 46 Publications. Collapse Publications.

Array 496-EB

• Ephrin-B2 Antibodies
• Ephrin-B2 ELISA Kit
• Ephrin-B2 Lysate
• Ephrin-B2 Proteins
• Ephrin-B2 Proteins and Enzymes