Reactivity | VSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Reaction conditions will need to be optimized for each specific application. We recommend an initial MERS-CoV 3CL Protease concentration of 50-100 nM when using SARS CoV-2 3CL Protease Substrate, Rh110-conjugated (Catalog # S-720). |
Source | E. coli-derived mers-cov 3CL Protease protein Ser1 - Gln306 (Val304Leu & Met305Phe) with a C-terminal 6-His tag |
Accession # | |
Protein/Peptide Type | Recombinant Enzymes |
Purity | >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Dilutions |
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Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Supplied as a solution in HEPES, NaCl, DTT and EDTA. |
Purity | >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
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