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Recombinant MERS-CoV 3CL Protease, CF

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Product Details

Summary
Reactivity VSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant MERS-CoV 3CL Protease, CF Summary

Details of Functionality
Reaction conditions will need to be optimized for each specific application. We recommend an initial MERS-CoV 3CL Protease concentration of 50-100 nM when using SARS CoV-2 3CL Protease Substrate, Rh110-conjugated (Catalog # S-720).
Source
E. coli-derived mers-cov 3CL Protease protein
Ser1 - Gln306 (Val304Leu & Met305Phe) with a C-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant MERS-CoV 3CL Protease, CF

  • 3CL Protease
  • 3CL Proteinase
  • 3C-like main protease
  • 3CL-Mpro
  • COVID-19
  • M Proteinase
  • Main Protease
  • Mpro

Background

The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human MERS-CoV coronavirus (Middle East Respiratory Syndrome coronavirus) is a C30-type cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide that cleaves proteins with sequences including a LQ[S/A/G] c-terminal to the glutamine residue.  3CL protease activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  
  1. Lu R. et al. (2015) Genome Announc. 3:e00818.
  2. Tomar S. et al. (2015) J. Biol. Chem. 290:19403.
  3. Wu A. et al. (2015) Virus Res. 208:56.

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Bioinformatics

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