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Recombinant Human IgG1 Fc Protein, CF

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Recombinant Human IgG1/Fc Protein (Catalog # 110-HG) has a molecular weight (MW) of 55.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a dimer. MW may differ from predicted MW due to post-translational ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Clonality
Polyclonal
Host
Human
Format
Carrier-Free
Concentration
LYOPH

Order Details

Recombinant Human IgG1 Fc Protein, CF Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived human IgG1 protein
IEGRMD Human IgG1
(Pro100-Lys330)
Substitutions: Leu117Ala, Leu118Glu, Gly120Ala, Thr276Ala
Accession # P01857.1
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile
Structure / Form
Dimer
Protein/Peptide Type
Recombinant Proteins
Clonality
Polyclonal
Host
Human
Gene
IGHG1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-35 kDa, reducing conditions
Publications
Read Publications using
110-HG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions

It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IgG1 Fc Protein, CF

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Publications for IgG1 (110-HG)(127)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, N/A, T. gondii, Transgenic Mouse, Xenopus.

We have publications tested in 16 applications: Affinity Purification, Bioassay, Cell Culture, Control, ELISA Capture, Flow Cytometry, Flow Cytometry Control, Functional ELISA, IHC-P, Immunoprecipitation, In Vivo, In vivo assay, Isotype Control, Neutralization Control, Reference Standard, Surface Plasmon Resonance.


Filter By Application
Affinity Purification
(1)
Bioassay
(55)
Cell Culture
(5)
Control
(35)
ELISA Capture
(2)
Flow Cytometry
(1)
Flow Cytometry Control
(3)
Functional ELISA
(1)
IHC-P
(1)
Immunoprecipitation
(2)
In Vivo
(10)
In vivo assay
(1)
Isotype Control
(2)
Neutralization Control
(1)
Reference Standard
(2)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(68)
Mouse
(42)
Rat
(10)
Bovine
(1)
N/A
(2)
T. gondii
(1)
Transgenic Mouse
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 127. Show All 127 Publications.
Publications using 110-HG Applications Species
Cho, S;Servián-Morilla, E;Garrido, VN;Rodriguez-Gonzalez, B;Yuan, Y;Cano, R;Rambhiya, AA;Darabi, R;Haltiwanger, RS;Paradas, C;Jafar-Nejad, H; The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice bioRxiv : the preprint server for biology 2024-11-25 [PMID: 39651163] (Bioassay, Mouse) Bioassay Mouse
Alvarez, JA;Gas-Pascual, E;Malhi, S;Sánchez-Arcila, JC;Njume, FN;van der Wel, H;Zhao, Y;García-López, L;Ceron, G;Posada, J;Souza, SP;Yap, GS;West, CM;Jensen, KDC; The GPI sidechain of Toxoplasma gondii inhibits parasite pathogenesis mBio 2024-09-20 [PMID: 39302131] (Bioassay, T. gondii) Bioassay T. gondii
Guo, S;Wang, Y;Duan, Q;Gu, W;Fu, Q;Ma, Z;Ruan, J; Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation Frontiers in pharmacology 2024-09-03 [PMID: 39290870] (In vivo assay, Mouse) In vivo assay Mouse
Liu, T;Wu, G;Gudd, CLC;Trovato, FM;Barbera, T;Liu, Y;Triantafyllou, E;McPhail, MJW;Thursz, MR;Khamri, W; Cis-interaction between CD52 and T cell receptor complex interferes with CD4+ T cell activation in acute decompensation of cirrhosis EBioMedicine 2024-09-13 [PMID: 39276679] (Bioassay, Human) Bioassay Human
Stewen, J;Kruse, K;Godoi-Filip, AT;Zenia, ;Jeong, HW;Adams, S;Berkenfeld, F;Stehling, M;Red-Horse, K;Adams, RH;Pitulescu, ME; Eph-ephrin signaling couples endothelial cell sorting and arterial specification Nature communications 2024-04-03 [PMID: 38570531] (Bioassay, Human) Bioassay Human
Rhymes, ER;Simkin, RL;Qu, J;Villarroel-Campos, D;Surana, S;Tong, Y;Shapiro, R;Burgess, RW;Yang, XL;Schiavo, G;Sleigh, JN; Boosting BDNF in muscle rescues impaired axonal transport in a mouse model of DI-CMTC peripheral neuropathy bioRxiv : the preprint server for biology 2024-03-11 [PMID: 38559020] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Martins, C;Rasbach, E;Heppt, MV;Singh, P;Kulcsar, Z;Holzgruber, J;Chakraborty, A;Mucciarone, K;Kleffel, S;Brandenburg, A;Hoetzenecker, W;Rahbari, NN;DeCaprio, JA;Thakuria, M;Murphy, GF;Ramsey, MR;Posch, C;Barthel, SR;Schatton, T; Tumor cell-intrinsic PD-1 promotes Merkel cell carcinoma growth by activating downstream mTOR-mitochondrial ROS signaling Science advances 2024-01-19 [PMID: 38241371] (Bioassay, Human) Bioassay Human
Tsai, CH;Liu, E;Phan, A;Lu, KL;Mei, H; NBL1 Reduces Corneal Fibrosis and Scar Formation after Wounding Biomolecules 2023-10-24 [PMID: 38002252] (Bioassay, Human, Mouse) Bioassay Human, Mouse
Erlichman, N;Meshel, T;Baram, T;Abu Raiya, A;Horvitz, T;Ben-Yaakov, H;Ben-Baruch, A; The Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Breast Cancer Are Regulated by N-Linked Glycosylation-Dependent Activation of STAT3 and STAT1 Cells 2023-09-23 [PMID: 37830552] (Bioassay, Human) Bioassay Human
Mellergaard, M;Skovbakke, SL;Jepsen, SD;Panagiotopoulou, N;Hansen, ABR;Tian, W;Lund, A;Høgh, RI;Møller, SH;Guérillot, R;Hayes, AS;Erikstrup, LT;Andresen, L;Peleg, AY;Larsen, AR;Stinear, TP;Handberg, A;Erikstrup, C;Howden, BP;Goletz, S;Frees, D;Skov, S; Clinical Staphylococcus aureus inhibits human T-cell activity through interaction with the PD-1 receptor mBio 2023-10-05 [PMID: 37796131] (Flow Cytometry Control, Human) Flow Cytometry Control Human
Show All 127 Publications.

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Bioinformatics

Gene Symbol IGHG1
Uniprot