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Recombinant Human IgG1 Fc Protein, CF

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Recombinant Human IgG1/Fc Protein (Catalog # 110-HG) has a molecular weight (MW) of 55.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a dimer. MW may differ from predicted MW due to post-translational ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Clonality
Polyclonal
Host
Human
Format
Carrier-Free
Concentration
LYOPH

Order Details

Recombinant Human IgG1 Fc Protein, CF Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived human IgG1 protein
IEGRMD Human IgG1
(Pro100-Lys330)
Substitutions: Leu117Ala, Leu118Glu, Gly120Ala, Thr276Ala
Accession # P01857.1
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile
Structure / Form
Dimer
Protein/Peptide Type
Recombinant Proteins
Clonality
Polyclonal
Host
Human
Gene
IGHG1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-35 kDa, reducing conditions
Publications
Read Publications using
110-HG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions

It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IgG1 Fc Protein, CF

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Publications for IgG1 (110-HG)(121)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, N/A, Transgenic Mouse, Xenopus.

We have publications tested in 15 applications: Affinity Purification, Bioassay, Cell Culture, Control, ELISA Capture, Flow Cytometry, Flow Cytometry Control, Functional ELISA, IHC-P, Immunoprecipitation, In Vivo, Isotype Control, Neutralization Control, Reference Standard, Surface Plasmon Resonance.


Filter By Application
Affinity Purification
(1)
Bioassay
(51)
Cell Culture
(5)
Control
(35)
ELISA Capture
(2)
Flow Cytometry
(1)
Flow Cytometry Control
(3)
Functional ELISA
(1)
IHC-P
(1)
Immunoprecipitation
(1)
In Vivo
(10)
Isotype Control
(2)
Neutralization Control
(1)
Reference Standard
(2)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(66)
Mouse
(39)
Rat
(10)
Bovine
(1)
N/A
(2)
Transgenic Mouse
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 121. Show All 121 Publications.
Publications using 110-HG Applications Species
Martins, C;Rasbach, E;Heppt, MV;Singh, P;Kulcsar, Z;Holzgruber, J;Chakraborty, A;Mucciarone, K;Kleffel, S;Brandenburg, A;Hoetzenecker, W;Rahbari, NN;DeCaprio, JA;Thakuria, M;Murphy, GF;Ramsey, MR;Posch, C;Barthel, SR;Schatton, T; Tumor cell-intrinsic PD-1 promotes Merkel cell carcinoma growth by activating downstream mTOR-mitochondrial ROS signaling Science advances 2024-01-19 [PMID: 38241371] (Bioassay, Human) Bioassay Human
Tsai, CH;Liu, E;Phan, A;Lu, KL;Mei, H; NBL1 Reduces Corneal Fibrosis and Scar Formation after Wounding Biomolecules 2023-10-24 [PMID: 38002252] (Bioassay, Human, Mouse) Bioassay Human, Mouse
Erlichman, N;Meshel, T;Baram, T;Abu Raiya, A;Horvitz, T;Ben-Yaakov, H;Ben-Baruch, A; The Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Breast Cancer Are Regulated by N-Linked Glycosylation-Dependent Activation of STAT3 and STAT1 Cells 2023-09-23 [PMID: 37830552] (Bioassay, Human) Bioassay Human
Mellergaard, M;Skovbakke, SL;Jepsen, SD;Panagiotopoulou, N;Hansen, ABR;Tian, W;Lund, A;Høgh, RI;Møller, SH;Guérillot, R;Hayes, AS;Erikstrup, LT;Andresen, L;Peleg, AY;Larsen, AR;Stinear, TP;Handberg, A;Erikstrup, C;Howden, BP;Goletz, S;Frees, D;Skov, S; Clinical Staphylococcus aureus inhibits human T-cell activity through interaction with the PD-1 receptor mBio 2023-10-05 [PMID: 37796131] (Flow Cytometry Control, Human) Flow Cytometry Control Human
Lenza, MP;Egia-Mendikute, L;Antoñana-Vildosola, A;Soares, CO;Coelho, H;Corzana, F;Bosch, A;Manisha, P;Quintana, JI;Oyenarte, I;Unione, L;Moure, MJ;Azkargorta, M;Atxabal, U;Sobczak, K;Elortza, F;Sutherland, JD;Barrio, R;Marcelo, F;Jiménez-Barbero, J;Palazon, A;Ereño-Orbea, J; Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b Nature communications 2023-06-13 [PMID: 37311743] (Bioassay, Human) Bioassay Human
Li, X;Li, J;Zheng, Y;Lee, SJ;Zhou, J;Giobbie-Hurder, A;Butterfield, LH;Dranoff, G;Hodi, FS; Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Influence on Soluble and Membrane Bound ICOS in Combination with Immune Checkpoint Blockade Cancer immunology research 2023-06-01 [PMID: 37262321] (Neutralization Control, Human) Neutralization Control Human
Adel, SS;Clarke, VRJ;Evans-Strong, A;Maguire, J;Paradis, S; Semaphorin 4D induced inhibitory synaptogenesis decreases epileptiform activity and alters progression to Status Epilepticus in mice Epilepsy research 2023-04-27 [PMID: 37163910] (Bioassay, Mouse) Bioassay Mouse
CA Hokanson, E Zacco, G Cappuccill, T Odineca, R Crea AXL-Receptor Targeted 14FN3 Based Single Domain Proteins (Pronectins�) from 3 Synthetic Human Libraries as Components for Exploring Novel Bispecific Constructs against Solid Tumors Biomedicines, 2022-12-08;10(12):. 2022-12-08 [PMID: 36551940] (Bioassay, Mouse) Bioassay Mouse
S Tian, X Xiong, J Zeng, S Wang, BJ Tremblay, P Chen, B Chen, M Liu, P Chen, K Sheng, D Zeve, W Qi, DT Breault, C Rodríguez, R Gerhard, R Jin, AC Doxey, M Dong Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants Nature Communications, 2022-11-09;13(1):6786. 2022-11-09 [PMID: 36351897] (Reference Standard, Human) Reference Standard Human
K Szabó, I Jámbor, K Pázmándi, N Nagy, G Papp, T Tarr Altered Circulating Follicular T Helper Cell Subsets and Follicular T Regulatory Cells Are Indicators of a Derailed B Cell Response in Lupus, Which Could Be Modified by Targeting IL-21R International Journal of Molecular Sciences, 2022-10-13;23(20):. 2022-10-13 [PMID: 36293075] (Cell Culture, Human) Cell Culture Human
Show All 121 Publications.

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Bioinformatics

Gene Symbol IGHG1
Uniprot