Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein Summary
| Details of Functionality |
Measured by flow cytometry for its ability to bind anti-human IgG1 Fc Antibody conjugated beads. The concentration of Recombinant Human IgG1 Fc Alexa Fluor® 647 (Catalog # AFR110) that produces 50% of the binding response is 0.200-2.00 ng/mL. |
| Source |
Mouse myeloma cell line, NS0-derived human IgG1 protein | IEGRMD | Human IgG1
Pro100-Lys330
(Leu117Ala, Leu118Glu, Gly120Ala, Thr276Ala)
Accession # P01857.1 | |
| N-terminus | C-terminus | |
|
| Accession # |
|
| N-terminal Sequence |
Ile |
| Structure / Form |
Disulfide linked homodimer Labeled with Alexa Fluor® 647 Excitation
Wavelength: 650 nm Emission
Wavelength: 668 nm |
| Protein/Peptide Type |
Recombinant Proteins |
| Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note |
<1.0 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
| Theoretical MW |
26.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE |
30-40 kDa, under reducing conditions. |
Packaging, Storage & Formulations
| Storage |
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 6 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 3 months, -20 to -70 °C under sterile conditions after opening.
|
| Buffer |
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein
Background
Human Fc is the crystallizable fragment in the constant
region of the human immunoglobulin heavy chain.
Members of the immunoglobulin superfamily (IgSF) consist of two heavy
(H) and two light (L) chains, with each component containing one variable (V)
and one or more constant (C) domains (1, 2).
Produced by B lymphocytes, Ig gamma (IgG) is the predominant isotype
found in the body and has the longest half-life of all immunoglobulin isotypes
(3). Four IgG subclasses (IgG1-4) were
identified based on structural, antigenic and functional differences in the
constant region of the heavy chain. The constant tail of the antibody is where proteins
involved in immune responses typically bind, most notably the first component
of the complement pathway, C1q, and Fc receptors found on immune cells such as
B lymphocytes. Affinities
for C1q and Fc gamma receptor (Fc gamma R) differ
among the IgG subclasses. Fc receptors mediate the recruitment of immune cells to a
site of infection (4). Engineered crystallizable fragment (Fc) regions of
antibody which assume a unique and unprecedented asymmetric structure within
the homodimeric Fc polypeptide, enable completely selective binding to the
complement component C1q and activation of complement via the classical pathway
without any concomitant engagement of the Fc gamma R (5).
- Williams, A.F. and A.N. Barclay (1988) Annu. Rev. Immunol. 6:381.
- Harpaz, Y. and C. Chothia (1994) J. Mol. Biol. 238:528.
- Schroeder, H.W. and L. Cavacini (2010) J. Allergy Clin. Immunol. 125:S41.
- Duncan, A.R. and G. Winter (1998) Nature 332:738.
- Lee, C.H. et al. (2017) Nat. Immunol. 18:889.
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