Recombinant Human alpha 1B-Glycoprotein Protein, CF Summary
| Details of Functionality |
Measured by its binding ability in a functional ELISA. When Recombinant Human CRISP‑3 (Catalog # 2397-CR) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human alpha 1B‑Glycoprotein that produces 50% of the optimal binding response is approximately 0.4‑2.4 ng/mL. |
| Source |
Human embryonic kidney cell, HEK293-derived human alpha 1B-Glycoprotein protein
Ala22-Ser495, with a C-terminal 6-His tag |
| Accession # |
|
| N-terminal Sequence |
Starts at Ala22 |
| Structure / Form |
Monomer |
| Protein/Peptide Type |
Recombinant Enzymes |
| Gene |
A1BG |
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Endotoxin Note |
<0.01 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
52.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE |
70-80 kDa, reducing conditions |
Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human alpha 1B-Glycoprotein Protein, CF
Background
Alpha-1B-glycoprotein (A1BG) is an approximately 80 kDa molecule that circulates in the serum (1). Mature human A1BG consists of five tandem Ig‑like domains and shares 44% amino acid sequence identity with mouse and rat A1BG (2, 3). Alternative splicing generates an isoform that lacks the signal peptide and the first Ig‑like domain. A1BG binds with high affinity to the cysteine‑rich protein CRISP‑3 (4). In pancreatic carcinoma, it is elevated in pancreatic juice as well as in the serum relative to normal patients (5, 6). A1BG is abnormally excreted into the urine during idiopathic nephrotic syndrome (INS) (7). Multiple breakdown fragments of A1BG are also present in INS urine, including one of 14 kDa that is found in steroid‑resistant but not steroid‑sensitive cases (7). A1BG is also elevated in the urine of patients with interstitial cystitis/painful bladder syndrome (IC/PBS) (8).
- Schultze, H.E. et al. (1963) Nature 200:1103.
- Ishioka, N. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2363.
- Gardmo, C. et al. (2001) Endocrinology 142:2695.
- Udby, L. et al. (2004) Biochemistry 43:12877.
- Tian, M. et al. (2008) BMC Cancer 8:241.
- Li, C. et al. (2009) J. Proteome Res. 8:483.
- Piyaphanee, N. et al. (2011) Proteomics Clin. Appl. 5:334.
- Goo, Y.A. et al. (2010) Int. Braz. J. Urol. 36:464.
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