| Reactivity | Pm-CmSpecies Glossary |
| Applications | Bioactivity |
| Format | Carrier-Free |
| Details of Functionality | Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey IgG1 Fc is immobilized at 0.15 µg/mL
(100 µL/well), the concentration of
Recombinant
Human Fc gamma RI/CD64 (Catalog # 1257-FC)
that produces 50% of the optimal binding response is 0.2-1.2 ng/mL. |
| Source | Human embryonic kidney cell, HEK293-derived cynomolgus monkey IgG1 protein Pro110-Lys333 |
| Accession # | |
| N-terminal Sequence | Pro110, and Ala-Leu-Ala-Pro110 |
| Protein/Peptide Type | Recombinant Proteins |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
|
| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE | 30-40 kDa, reducing conditions |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Reconstitution Instructions | Reconstitute at 1 mg/mL in PBS. |
|
Read full blog post. |
|
CD16 - Find me on macrophages, neutrophils and NK cells CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells.... Read full blog post. |
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