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Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF

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When Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 μg/mL, 100 μL/well, Recombinant Human Fc gamma RI/CD64 (Catalog # 1257-FC) binds with an ED50 of 0.2-1.2 ng/mL.
When Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 μg/mL, 100 μL/well, Recombinant Human Fc gamma RI/CD64 (Catalog # 1257-FC) binds with an ED50 of 0.2-1.2 ng/mL.

Product Details

Summary
Reactivity Pm-CmSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey IgG1 Fc is immobilized at 0.15 µg/mL (100 µL/well), the concentration of Recombinant Human Fc gamma  RI/CD64 (Catalog # 1257-FC) that produces 50% of the optimal binding response is 0.2-1.2 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey IgG1 protein
Pro110-Lys333
Accession #
N-terminal Sequence
Pro110, and Ala-Leu-Ala-Pro110
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-40 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in human adult blood. It is composed of two heavy chains and two light chains. The variability of the IgG pool is generated by somatic recombination in B cells that have been exposed to foreign material. IgA, IgG, IgM, IgD, and IgD classes of human immunoglobulin (as well as the isotypes IgG1, IgG2a, IgG2b, and IgG3) are generated by class switch recombination within the heavy chain locus (1). Recombination within the light chain locus selects either kappa or lambda chains. Antibodies are secreted by plasma cells of the B lymphocyte lineage and constitute the humoral component of adaptive immunity (2, 3). Their variable regions bind to foreign antigens, while their Fc regions are recognized by Fc receptors expressed on macrophages, monocytes, neutrophils, NK cells, and CD8+ cytotoxic T cells (4). Phagocyte uptake of antibody-antigen complexes and antibody-opsonized pathogens is critical for clearance of the foreign material and immune homeostasis.
  1. Stavnezer, J. et al. (2008) Annu. Rev. Immunol. 26:261.
  2. Nutt, S.L. et al. (2015) Nat. Rev. Immunol. 15:160.
  3. Swanson, C.L. et al. (2013) Immunol. Res. 55:277.
  4. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.

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