Raw 264.7 Whole Cell - T0901317 treated Assay Kit Summary
| Description |
Protein supplied in 1X Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 10% prior to use. NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Samples should be heated to 65C for 15 minutes prior to loading. |
| Kit Type |
Assay Kit |
Applications/Dilutions
| Dilutions |
- Western Blot 0.01 mL per lane
|
Packaging, Storage & Formulations
| Storage |
Store at -20 degrees C. Avoid freeze/thaw cycles. |
Details for Array
| Type |
Cell |
| Life Stage |
Embryonic |
| Tissue |
Blood Lymph |
| Protein State |
Denatured |
| Tissue Condition |
Tumor |
| Subcellular Fraction |
Whole |
Kit Components
Background
T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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![Western Blot: Raw 264.7 Whole Cell - T0901317 treated Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).](http://images.novusbio.com/fullsize/Raw-264.7-Whole-Cell-T0901317-treated-Kit-Western-Blot-NBP1-30159-img0003.jpg "Western Blot: Raw 264.7 Whole Cell - T0901317 treated Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).")
