Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow™ Summary
| Description |
Rainbow Calibration Particles are a mixture of fluorophore incorporated particles with 8 different fluorescent intensities that are used for calibrating flow cytometers:
- Excite particles at any wavelength from 365 to 650 nm.
- Calibrate multiple channels with the same set of particles.
- Enable non-spectral matching to common fluorophores such as FITC, PE, PE-Cy5.
- Deliver durable performance since fluorophores are encased within particles, not on the surface.
- Remain stable for several years.
- Packaged in a dropper bottle for easy dispensing and storage.
Concentration: 1 x 10^7 particles / mL
Particle size: 3.0 - 3.4 micron
Particle material: polystyrene
Uses: Long term performance tracking or routine calibration of flow cytometers, specific for following the EuroFlow(TM) standardization guidelines. Routine alignment and calibration in fluorescence and confocal fluorescence microscopy. |
Applications/Dilutions
| Dilutions |
- Flow Cytometry
- Fluorescence Imaging
|
| Application Notes |
Shake vigorously or vortex briefly before use. Inclusion of a small amount of detergent in the diluent will help increase the number of singles. Diluted particles can be stored at 4C for future use. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. Do not freeze. |
| Buffer |
Deionized water, 0.01% NP-40 |
| Preservative |
0.02% Sodium Azide |
Alternate Names for Rainbow Calibration Particle Set (8 peaks - EAL01) - EuroFlow(TM)
Background
Rainbow Calibration Particles are used to ascertain the relative voltage range for each flow cytometer detector. This mixture of fluorescent particles serves as a reliable and reproducible means of evaluating the sensitivity and resolution of flow cytometers.
The relative number of fluorophores can been determined for every emission peak of the rainbow calibration particles in FL1 (FITC, MEFL), FL2 (RPE, MEPE), FL3 (RPE-Cy5, MEPCY) and FL4 (APC, MEAP) channels in order to plot the calibration graph. The calibration graph is used to check the linearity (dynamic range) of the photomultiplier tube (PMT) in each channel. Additionally, the relative number of fluorophores can be cross calibrated with cells or particles stained with a known number of spectral matching fluorophores such as FITC, PE, RPE-Cy5 to estimate the number of fluorophores on stained cells.
Rainbow Calibration Particle Set (8 peaks - EAL01) are used in studies following EuroFlow(TM) standard guidelines for PMT characterization, to set target mean fluorescence intensities (MFI) values and for daily performance tracking of flow cytometers. They provide target MFIs for the standardization of an 8-color flow cytometric immunophenotyping panel of normal and malignant leukocytes in bone marrow and blood (1).
References
1. Kalina, T., Flores-Montero, J., van der Velden V.H.J., Martin-Ayuso, M., Bottcher, S., Ritgen, M., Almeida, J., Lhermitte,L., Asnafi, V., Mendonca, A., de Tute, R., Cullen, M., Sedek, L., Vidriales, M.B., Perez, J.J., te Marvelde, J.G., Mejstrikova, E., Hrusak, O., Szczepanski, T., van Dongen, J.J.M., and Orfao A.. (2012). EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 26(9):1986-2010. https://doi.org/10.1038/leu.2012.122
EuroFlow(TM) is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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![Rainbow Calibration Particle Set (8 peaks - EAL01) [NBP3-00506] - Dot Plot and histograms for NBP3-00506 on a BD Biosciences LSRFortessa(TM) X-20 using assigned target values.](http://images.novusbio.com/fullsize/Rainbow-Calibration-Particle-Set-8-peaks-EAL01-NBP3-00506-img0001.jpg "Rainbow Calibration Particle Set (8 peaks - EAL01) [NBP3-00506] - Dot Plot and histograms for NBP3-00506 on a BD Biosciences LSRFortessa(TM) X-20 using assigned target values.")
