| Reactivity | HuSpecies Glossary |
| Description | Normal Tissue LysateDiagnosis: Normal prostateSex: Male Age: 37 Grade: N/A Stage: N/A Normal Tissue Pathology Data Location: Prostate Gross findings: No data available Microscopic Findings No data available Preparation Method Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4 |
| Application Notes | These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets. For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required. Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays. These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
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![Immunohistochemistry: Human Prostate Tissue MicroArray (Cancer) [NBP2-30169] - Adenocarcinoma patient - Gleason score 7. RED: HDAC6. BLUE: DAPI, nucleus. IHC image submitted by a verified customer review.](https://images.novusbio.com/images/Human-Prostate-Tissue-MicroArray-Cancer-Immunohistochemistry-NBP2-30169-img0100.jpg "Immunohistochemistry: Human Prostate Tissue MicroArray (Cancer) [NBP2-30169] - Adenocarcinoma patient - Gleason score 7. RED: HDAC6. BLUE: DAPI, nucleus. IHC image submitted by a verified customer review.")