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Chicken Progesterone ELISA Kit (Colorimetric)

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Chicken Progesterone ELISA Kit (Colorimetric) [NBP3-23550] - Samples were spiked with high concentrations of Chicken Progesterone and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Chicken Progesterone ELISA Kit (Colorimetric) [NBP3-23550] - Standard Curve Reference

Product Details

Summary
Reactivity ChSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.06 ng/mL

Order Details

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Chicken Progesterone ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Chicken Progesterone in samples. No significant cross-reactivity or interference between Chicken Progesterone and analogues was observed.
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.06 ng/mL
Assay Type
Competitive-ELISA
Inter-Assay
%CV < 5.57
Intra-Assay
%CV < 5.76
Spike Recovery
89-104%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Concentrated HRP Linked Detection Ab (100x)
  2. Concentrated Wash Buffer (25x)
  3. HRP Linked Ab Diluent
  4. Micro ELISA Plate (Dismountable)
  5. Plate Sealer
  6. Product Manual
  7. Reference Standard
  8. Sample Diluent
  9. Stop Solution
  10. Substrate Reagent

Alternate Names for Chicken Progesterone ELISA Kit (Colorimetric)

  • 4-Pregnen-3,20-dione
  • Progesterone

Background

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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