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Bovine Progesterone ELISA Kit (Colorimetric)

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ELISA: Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122] - Standard Curve Reference

Product Details

Summary
Reactivity BvSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
0.15 ng/mL - 70 ng/mL
Sensitivity
0.2 ng/mL

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Bovine Progesterone ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of bovine PROG. No significant cross-reactivity or interference between bovine PROG and analogues was observed.
Standard Curve Range
0.15 ng/mL - 70 ng/mL
Sensitivity
0.2 ng/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using NBP2-60122.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Cover Strip (for 96 wells)
  2. Antibody
  3. Assay plate
  4. HRP-conjugate
  5. Instruction manual
  6. Standard
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer (20X concentrate)

Alternate Names for Bovine Progesterone ELISA Kit (Colorimetric)

  • 4-Pregnen-3,20-dione
  • Progesterone

Background

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PROG and Horseradish Peroxidase (HRP) conjugated PROG. The competitive inhibition reaction is launched between with HRP labeled PROG and unlabeled PROG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PROG in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Progesterone ELISA Kit (NBP2-60122)(3)

We have publications tested in 1 application: ELISA.


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Product General Protocols

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