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Phospho-Tyrosine Antibody (179003) [Agarose]

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A431 human epithelial carcinoma cell line was untreated (-) or treated (+) with 200 mM pervanadate (PV) for 15 minutes. Phospho-tyrosine was immunoprecipitated from 500 µg of cell lysate following incubation with 10 µg Mouse Anti-Phospho-Tyrosine Agarose-conjugated Monoclonal Antibody (Catalog # AGM1676) for 16 hours at 2 - 8 ºC. Immunoprecipitated phospho-tyrosine was detected by Western blot using 1 µg Phospho-Tyrosine Biotinylated Monoclonal Antibody (left panel, Catalog # BAM1676) or Human EGF R/ErbB1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (right panel, Catalog # BAF231). View our recommended buffer recipes for immunoprecipitation.
A431 human epithelial carcinoma cell line was untreated (-) or treated (+) with 200 mM pervanadate (PV) for 15 minutes. Phospho-tyrosine was immunoprecipitated from 500 µg of cell lysate following incubation with 10 ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications IP
Clone
179003
Clonality
Monoclonal
Host
Mouse
Conjugate
Agarose

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alkaline Phosphatase
APM1676
Biotin
BAM1676
HRP
HAM1676
Unconjugated
MAB1676-100
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1676-100
Carrier Free
MAB1676-SP
Carrier Free
MAB1676-500
Carrier Free
MAB1676-100
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Phospho-Tyrosine Antibody (179003) [Agarose] Summary

Specificity
Detects endogenous proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using ligand- and pervanadate-treated cell lysates indicate that clone 179003 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 10 uL/500 ug cell lysate

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Phosphate-buffered saline (PBS) with preservative.
Preservative
No Preservative
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospho-Tyrosine Antibody (179003) [Agarose]

  • 2 amino 3(4 hydroxyphenyl) propanoic acid
  • 4 hydroxyphenylalanine
  • PhosphoTyrosine
  • pTyrosine
  • pY20
  • Tyrosine

Background

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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