![Western Blot: PGAM1/2/4 Antibody [NB100-774] - staining of Mouse (A), Rat (B) and Pig (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PGAM1-2-4-Antibody-Western-Blot-NB100-774-img0002.jpg "Western Blot: PGAM1/2/4 Antibody [NB100-774] - staining of Mouse (A), Rat (B) and Pig (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence according to NP_002620.1; NP_000281.2; NP_001025062.1. |
| Specificity | Please note this antibody is expected to recognize the products of 3 highly similar genes. |
| Predicted Species | Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PGAM1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx 27kDa band observed in Human Cerebellum and in Human and Mouse Liver lysates, and approx. 28kDa band observed in Rat and Pig Liver lysates (calculated MW of 28.8kDa according to Human NP_002620.1, NP_000281.2, and NP_001025062.1, Mouse NP_075907.2, Rat NP_445742.2 and Pig XP_003483583.1 ). |
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| Theoretical MW | 29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Human Liver Whole Tissue Lysate (Adult Whole Normal) | |
| PGAM1 Overexpression Lysate | |
| Human Brain Whole Tissue Lysate (Adult Whole Normal) |
Secondary Antibodies |
Isotype Controls |
Research Areas for PGAM1/2/4 Antibody (NB100-774)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Subcellular localization of PGAM with the use of antibody directed to C-terminal peptide of the proteinThe localization was examined in cultures of non-small cell lung carcinoma (NSCLC) HL-1 cardiomyocytes, astrocytes and in breast cancer tissue section (BC). Arrows point nucleoli. Bar=5 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.4044), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PGAM1-2-4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-774-img0004.jpg "Immunocytochemistry/Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Subcellular localization of PGAM with the use of antibody directed to C-terminal peptide of the proteinThe localization was examined in cultures of non-small cell lung carcinoma (NSCLC) HL-1 cardiomyocytes, astrocytes and in breast cancer tissue section (BC). Arrows point nucleoli. Bar=5 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.4044), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Localization of PGAM with antibody directed to the C-terminal peptide of the protein -in nuclei of KLN-205 cells treated with drugs disturbing ribosomal biogenesisActD = actinomycin D; PI - propidium iodide. Bar=5 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.4044), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PGAM1-2-4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-774-img0003.jpg "Immunocytochemistry/Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Localization of PGAM with antibody directed to the C-terminal peptide of the protein -in nuclei of KLN-205 cells treated with drugs disturbing ribosomal biogenesisActD = actinomycin D; PI - propidium iodide. Bar=5 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.4044), licensed under a CC-BY license.")
![Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.](https://images.novusbio.com/images/Human-Brain-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59177-img0001.jpg "Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.")
