Applications/Dilutions
| Application Notes |
The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture |
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA) |
| Concentration |
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance. |
Alternate Names for pCLNRX Retrovirus Expression Vector
Background
The pCLNCX expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safetymodified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation (1), thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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![pCLNRX Retrovirus Expression Vector [NBP2-29538] - Schematic presentation of pCLXSN vector: CMV immediate early enhancer-promoter allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI, Xho I, or Bam HI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5).](http://images.novusbio.com/fullsize/pCLNRX-Retrovirus-Expression-Vector-NBP2-29538-img0001.jpg "pCLNRX Retrovirus Expression Vector [NBP2-29538] - Schematic presentation of pCLXSN vector: CMV immediate early enhancer-promoter allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI, Xho I, or Bam HI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5).")
