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PC-12 Whole Cell Lysate

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

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PC-12 Whole Cell Lysate Summary

Specificity
PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (non-denatured)

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot
Application Notes
This product is not supplied in loading buffer. If you intend to us this product in Western Blot, we recommend 1 x sample buffer with 5% 2-Mercaptoethanol (BME) or other reducing agent.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Standard RIPA buffer including protease and phosphotase inhibitors and 0.1% SDS
Preservative
No Preservative
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Nervous
Protein State
Native
Subcellular Fraction
Whole

Background

PC12 is a cell line derived from a pheochromocytoma of a male rat adrenal gland. PC12 cells are small, irregularly shaped cells that form as floating clusters with a few scattered attached cells in culture. PC12 cells are also suitable as a transfection host. With the addition of nerve growth factor to PC12 cells, they will stop dividing and terminally differentiate, making PC12 a useful model for neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional PC-12 Products

Array NBP2-13170

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