PC-12 Whole Cell Lysate Summary
Specificity |
PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (non-denatured) |
Applications/Dilutions
Dilutions |
- Immunoprecipitation
- Western Blot
|
Application Notes |
This product is not supplied in loading buffer. If you intend to us this product in Western Blot, we recommend 1 x sample buffer with 5% 2-Mercaptoethanol (BME) or other reducing agent. |
Packaging, Storage & Formulations
Storage |
Store at -80C. Avoid freeze-thaw cycles. |
Buffer |
Standard RIPA buffer including protease and phosphotase inhibitors and 0.1% SDS |
Preservative |
No Preservative |
Concentration |
5.0 mg/ml |
Lysate Details for Array
Type |
Cell |
Tissue |
Nervous |
Protein State |
Native |
Subcellular Fraction |
Whole |
Background
PC12 is a cell line derived from a pheochromocytoma of a male rat adrenal gland. PC12 cells are small, irregularly shaped cells that form as floating clusters with a few scattered attached cells in culture. PC12 cells are also suitable as a transfection host. With the addition of nerve growth factor to PC12 cells, they will stop dividing and terminally differentiate, making PC12 a useful model for neuronal differentiation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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