NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

Placental alkaline phosphatase (PLAP) is one of the most stable isoenzymes, only existing in the placenta of higher primates. These characteristics make placental alkaline phosphatase suitable to use as a reporter gene for the analysis of promoter activity and gene expression in cell culture and animal serum. The natural form of PLAP is membrane anchored. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function. SEAP is designed by inserting a translational terminator after amino acid 489 (Berger, et al., Gene 66 (1): 10 (1988). This mutation converts the membrane-bound PLAP protein into the secreted protein SEAP. As a major transcription factor NF-KB plays a key role in regulating genes responsible for the innate and adaptive immune responses. In unstimulated cells, the NF-KB dimers are held in cytoplasm by IKBs that masks the nuclear localization signals of NF-KB. Upon cell stimulation, which leads to IKB degradation, NF-KB quickly translocates to the nucleus and activates various genes that have DNA-binding sites for NF-KB. Our NF-KB SEAPORTER Assay Kit is designed to measure NF-KB activation using SEAP protein secreted to the culture media as a read-out.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ELISA.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using NBP2-25286	Applications	Species
Marwarha G, Slagsvold KH, Høydal MA NF-?B Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression International journal of molecular sciences 2023-04-01 [PMID: 37047592]
Wang RW, ViganO S, Ben-David U et al. Aneuploid senescent cells activate NF-kappa B to promote their immune clearance by NK cells EMBO reports 2021-06-08 [PMID: 34105235]
Lai L, Yang G, Yao X et al. NLRC4 Mutation in flagellin-derived peptide CBLB502 ligand-binding domain reduces the inflammatory response but not radioprotective activity J. Radiat. Res. 2019-10-10 [PMID: 31599956] (Human)		Human
Fereig RM, Abdelbaky HH, Kuroda Y, Nishikawa Y. Critical role of TLR2 in triggering protective immunity with cyclophilin entrapped in oligomannose-coated liposomes against Neospora caninum infection in mice Vaccine 2019-02-08 [PMID: 30660401] (Mouse)		Mouse
Manzo Emiliano, Cutignano Adele, Pagano Dario et al. A new marine-derived sulfoglycolipid triggers dendritic cell activation and immune adjuvant response. Scientific Reports 2017-01-01 [PMID: 28740080]
Toshimitsu T, Ozaki S, Mochizuki J et al. Effects of Lactobacillus plantarum OLL2712 culture conditions on the anti-inflammatory activity for murine immune cells and obese and type 2 diabetic mice Appl. Environ. Microbiol. 2017-01-13 [PMID: 28087537]
O'Neill S, Humphries D, Tse G et al. Heat shock protein 90 inhibition abrogates TLR4-mediated NF-kB activity and reduces renal ischemia-reperfusion injury. Sci Rep. 2015-08-07 [PMID: 26248657] (ELISA)	ELISA
Chen J, Ng MM, Chu JJ. Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection. PLoS Pathog. 2015-07-01 [PMID: 26226614] (ELISA) Details: MALP-2, TLR6 and TLR2 ligand was used at 50 ng/ml concentration (alongwith mock or Dengue Virus DV2-infection and LPS ) for treatments of human PBMCs, HEK293 and wild-type or TLR6-/- murine peritoneal macrophages under various experimental conditions.	ELISA
Hayashi T, Yao S, Crain B et al. Induction of Tolerogenic Dendritic Cells by a PEGylated TLR7 Ligand for Treatment of Type 1 Diabetes PLoS ONE. 2015-06-16 [PMID: 26076454] (ELISA, Human)	ELISA	Human
McKim JM, Wilga PC, Pregenzer JF, Blakemore WR. The common food additive carrageenan is not a ligand for toll-like- receptor 4 (TLR4) in an HEK293-TLR4 reporter cell-line model Food Chem. Toxicol. 2015-01-29 [PMID: 25640528] (ELISA) Details: Human TLR4/MD-2/CD14 SEAP - (SEAPorterTM) Stable Reporter Cell Line [HEK293 cells stably transfected with a TLR4/MD-2/CD14/NF-kB/SEAP reporter construct - expresses full-length human TLR4, MD-2, CD14, and secreted alkaline phosphatase/SEAP under the control of an NFkB response element] used for evaluating the ability of different types of Carrageenan/CGN to bind and activate TLR4 signaling. SEAP was measured as an indicator of TLR4 activation with SEAPorterTM assay kit and CGN was found to not to activate the TLR4.	ELISA
Zurcher C, Sauter KS, Schweizer M. Pestiviral Erns blocks TLR-3-dependent IFN synthesis by LL37 complexed RNA. Veterinary Microbiology. 2014-10-13 [PMID: 25457366] (ELISA)	ELISA
Signorino G, Mohammadi N, Patane F et al. Role of TLR13 in innate immune recognition of group B streptococci. Infect. Immun. 2014-09-15 [PMID: 25225249] (ELISA, Human) Details: Human NFkB Secreted Alkaline Phosphatase SEAP - (SEAPorter™) Stable Reporter Cell Line was transiently transfected with full-length mouse TLR13 and stimulated with heat-killed bacteria and bacterial RNA or with phorbol myristate acetate /PMA followed by quantification of NFkB Secreted Alkaline Phosphatase using ELISA kit for the latter.	ELISA	Human
Wang RW A Mechanistic Evaluation of the Role of Aneuploidy During Oncogenesis Thesis 2022-01-01
Rajasekaran V. Tissue Transglutaminase (TG2) is a potential therapeutic target in the treatment of chemoresistant breast cancer. Breast Cancer Res 2012-01-10 [PMID: 22225906]
Show All 14 Publications. Collapse Publications.

Array NBP2-25286

• NFkB Secreted Alkaline Phosphatase Reporter Kit